Highly Efficient Biosynthesis of Nicotinic Acid by Immobilized Whole Cells of <i>E. coli</i> Expressing Nitrilase in Semi-Continuous Packed-Bed Bioreactor
A recombinant <i>E. coli</i>, expressing nitrilase from <i>Acidovorax facilis</i> 72W with dual-site expression plasmid pRSFduet (<i>E. coli</i> pRSF-AfNit2), was constructed. It showed higher soluble expression of nitrilase than that in the pET21a plasmid. The re...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | Catalysts |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4344/13/2/371 |