Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging

Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging

Summary: Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vi...

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Bibliographic Details
Main Authors: Matthew J. Ford, Keerthana Harwalkar, Yojiro Yamanaka
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell isolation
Developmental biology
Cancer
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000442

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http://www.sciencedirect.com/science/article/pii/S2666166722000442

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