Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging
Summary: Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722000442 |
| _version_ | 1818775414272688128 |
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| author | Matthew J. Ford Keerthana Harwalkar Yojiro Yamanaka |
| author_facet | Matthew J. Ford Keerthana Harwalkar Yojiro Yamanaka |
| author_sort | Matthew J. Ford |
| collection | DOAJ |
| description | Summary: Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vitro tool to study epithelial homeostasis and malignant transformation. We also outline a protocol for whole-mount immunofluorescence and 3D confocal imaging. In addition, we describe approaches of viral transduction to investigate gene function in organoid development and epithelial cell behavior.For complete details on the use and execution of this profile, please refer to Ford et al. (2021). |
| first_indexed | 2024-12-18T10:56:39Z |
| format | Article |
| id | doaj.art-1bcdd928ac694a5cb829fa76aae17751 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-18T10:56:39Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-1bcdd928ac694a5cb829fa76aae177512022-12-21T21:10:19ZengElsevierSTAR Protocols2666-16672022-03-0131101164Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imagingMatthew J. Ford0Keerthana Harwalkar1Yojiro Yamanaka2Rosalind and Morris Goodman Cancer Institute, McGill University, Department of Human Genetics, 1160 Pine Avenue West, Montreal, QC H3A 1A3, Canada; Corresponding authorRosalind and Morris Goodman Cancer Institute, McGill University, Department of Human Genetics, 1160 Pine Avenue West, Montreal, QC H3A 1A3, CanadaRosalind and Morris Goodman Cancer Institute, McGill University, Department of Human Genetics, 1160 Pine Avenue West, Montreal, QC H3A 1A3, Canada; Corresponding authorSummary: Epithelial cells lining the oviduct/fallopian tube are essential in reproduction and have been identified as the cell-of-origin in high-grade serous ovarian carcinoma (HGSOC). This protocol describes the generation of organoids from mouse oviduct epithelial cells, providing a powerful in vitro tool to study epithelial homeostasis and malignant transformation. We also outline a protocol for whole-mount immunofluorescence and 3D confocal imaging. In addition, we describe approaches of viral transduction to investigate gene function in organoid development and epithelial cell behavior.For complete details on the use and execution of this profile, please refer to Ford et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722000442Cell BiologyCell cultureCell isolationDevelopmental biologyCancerMicroscopy |
| spellingShingle | Matthew J. Ford Keerthana Harwalkar Yojiro Yamanaka Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging STAR Protocols Cell Biology Cell culture Cell isolation Developmental biology Cancer Microscopy |
| title | Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging |
| title_full | Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging |
| title_fullStr | Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging |
| title_full_unstemmed | Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging |
| title_short | Protocol to generate mouse oviduct epithelial organoids for viral transduction and whole-mount 3D imaging |
| title_sort | protocol to generate mouse oviduct epithelial organoids for viral transduction and whole mount 3d imaging |
| topic | Cell Biology Cell culture Cell isolation Developmental biology Cancer Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722000442 |
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