Biological nanopore approach for single‐molecule analysis of nucleobase modifications
Abstract Base modifications play an essential role in cellular function, and the abnormal expressions of base modifications are associated with numerous diseases. Unfortunately, existing detection methods have difficulty obtaining sequence information of various modified nucleobases at the single‐mo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2022-10-01
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| Series: | Electrochemical Science Advances |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsa.202100119 |
| _version_ | 1797741946134855680 |
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| author | Pengrui Lv Yongyi Yang Shuang Li Cherie S. Tan Dong Ming |
| author_facet | Pengrui Lv Yongyi Yang Shuang Li Cherie S. Tan Dong Ming |
| author_sort | Pengrui Lv |
| collection | DOAJ |
| description | Abstract Base modifications play an essential role in cellular function, and the abnormal expressions of base modifications are associated with numerous diseases. Unfortunately, existing detection methods have difficulty obtaining sequence information of various modified nucleobases at the single‐molecule resolution. Label‐free single‐molecule sequencing technology using biological nanopores can direct sequence canonical nucleobases. However, the discrimination of hundreds of noncanonical nucleobase modifications at the single‐molecule resolution is still challenging. In this minireview, we introduced the recent advances in detecting nucleobase modifications using biological nanopores from nucleic acid translocation controlling, confinement effects on nucleobase discrimination, and applications of nanopore sequencers for modification detection. |
| first_indexed | 2024-03-12T14:33:58Z |
| format | Article |
| id | doaj.art-1bf135bf4c4441519e09a72678ef3da0 |
| institution | Directory Open Access Journal |
| issn | 2698-5977 |
| language | English |
| last_indexed | 2024-03-12T14:33:58Z |
| publishDate | 2022-10-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Electrochemical Science Advances |
| spelling | doaj.art-1bf135bf4c4441519e09a72678ef3da02023-08-17T12:00:55ZengWiley-VCHElectrochemical Science Advances2698-59772022-10-0125n/an/a10.1002/elsa.202100119Biological nanopore approach for single‐molecule analysis of nucleobase modificationsPengrui Lv0Yongyi Yang1Shuang Li2Cherie S. Tan3Dong Ming4Academy of Medical Engineering and Translational Medicine Tianjin University Tianjin ChinaAcademy of Medical Engineering and Translational Medicine Tianjin University Tianjin ChinaAcademy of Medical Engineering and Translational Medicine Tianjin University Tianjin ChinaAcademy of Medical Engineering and Translational Medicine Tianjin University Tianjin ChinaAcademy of Medical Engineering and Translational Medicine Tianjin University Tianjin ChinaAbstract Base modifications play an essential role in cellular function, and the abnormal expressions of base modifications are associated with numerous diseases. Unfortunately, existing detection methods have difficulty obtaining sequence information of various modified nucleobases at the single‐molecule resolution. Label‐free single‐molecule sequencing technology using biological nanopores can direct sequence canonical nucleobases. However, the discrimination of hundreds of noncanonical nucleobase modifications at the single‐molecule resolution is still challenging. In this minireview, we introduced the recent advances in detecting nucleobase modifications using biological nanopores from nucleic acid translocation controlling, confinement effects on nucleobase discrimination, and applications of nanopore sequencers for modification detection.https://doi.org/10.1002/elsa.202100119biological nanoporenucleobase modificationsensorssingle‐molecule analysis |
| spellingShingle | Pengrui Lv Yongyi Yang Shuang Li Cherie S. Tan Dong Ming Biological nanopore approach for single‐molecule analysis of nucleobase modifications Electrochemical Science Advances biological nanopore nucleobase modification sensors single‐molecule analysis |
| title | Biological nanopore approach for single‐molecule analysis of nucleobase modifications |
| title_full | Biological nanopore approach for single‐molecule analysis of nucleobase modifications |
| title_fullStr | Biological nanopore approach for single‐molecule analysis of nucleobase modifications |
| title_full_unstemmed | Biological nanopore approach for single‐molecule analysis of nucleobase modifications |
| title_short | Biological nanopore approach for single‐molecule analysis of nucleobase modifications |
| title_sort | biological nanopore approach for single molecule analysis of nucleobase modifications |
| topic | biological nanopore nucleobase modification sensors single‐molecule analysis |
| url | https://doi.org/10.1002/elsa.202100119 |
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