A simple smiFISH pipeline to quantify mRNA at the single-cell level in 3D

A simple smiFISH pipeline to quantify mRNA at the single-cell level in 3D

Summary: Techniques allowing the precise quantification of mRNA at the cellular level are essential for understanding biological processes. Here, we present a semi-automated smiFISH (single-molecule inexpensive FISH) pipeline enabling quantification of mRNA in a small number of cells (∼40) in fixed...

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Bibliographic Details
Main Authors: Mathilde Bouchet, Séverine Urdy, Wenyue Guan, Chérif Kabir, Steve Garvis, Jonathan Enriquez
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cell Biology
Developmental biology
Microscopy
Molecular Biology
In Situ Hybridization
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002836

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http://www.sciencedirect.com/science/article/pii/S2666166723002836

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