A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts

A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts

Methylotrophic yeasts such as <i>Ogataea polymorpha</i> and <i>Komagataella phaffii</i> (sin. <i>Hansenula polymorpha</i> and <i>Pichia pastoris</i>, respectively) are commonly used in basic research and biotechnological applications, frequently those...

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Bibliographic Details
Main Authors: Azamat V. Karginov, Marina G. Tarutina, Anastasia R. Lapteva, Maria D. Pakhomova, Artur A. Galliamov, Sergey Y. Filkin, Alexey N. Fedorov, Michael O. Agaphonov
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:International Journal of Molecular Sciences
Subjects:
Cas9
CRISPR
genome engineering
genome editing
methylotrophic yeast
<i>Ogataea</i>
Online Access:https://www.mdpi.com/1422-0067/24/9/8173

Internet

https://www.mdpi.com/1422-0067/24/9/8173

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