Optimization and prospective evaluation of sensitive real-time PCR assays with an internal control for the diagnosis of melioidosis in Thailand

Optimization and prospective evaluation of sensitive real-time PCR assays with an internal control for the diagnosis of melioidosis in Thailand

ABSTRACT Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei. Effective treatment of melioidosis requires rapid detection of B. pseudomallei infection. Previous studies demonstrated that real-time PCR for the diagnosis of melioidosis in blood samples was insensitive. We op...

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Bibliographic Details
Main Authors: Chawitar Noparatvarakorn, Wallop Jakkul, Rathanin Seng, Sarunporn Tandhavanant, Orawan Ottiwet, Rachan Janon, Wadsamon Saikong, Narisara Chantratita
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:Microbiology Spectrum
Subjects:
Burkholderia pseudomallei
melioidosis
PCR
TTS1-orf2
BPSS0745
BPSS1187
Online Access:https://journals.asm.org/doi/10.1128/spectrum.01039-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.01039-23

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