Whole Spleen Flow Cytometry Assay
In the Whole Spleen Flow Cytometry Assay, we used splenocytes directly ex vivo for stimulation with a variety of TLR ligands. The splenocytes were stimulated for a total of 4 hours, then stained for intracellular cytokines. We then examined cytokine production via flow cytometry. This allowed us to...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2013-08-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=834&type=0 |