Protocols to monitor TFEB activation following lysosomal damage in cultured cells using microscopy and immunoblotting

Protocols to monitor TFEB activation following lysosomal damage in cultured cells using microscopy and immunoblotting

Summary: Following lysosomal damage, activation and nuclear translocation of transcription factor EB (TFEB) is the key event to maintain lysosomal homeostasis. Here, we describe steps to induce lysosomal damage in HeLa cells. This can be followed by monitoring the changes in TFEB localization using...

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Bibliographic Details
Main Authors: Monami Ogura, Takayuki Shima, Tamotsu Yoshimori, Shuhei Nakamura
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Antibody
Cell Biology
Cell culture
Cell separation/fractionation
Cell-based Assays
Microscopy
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007243

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http://www.sciencedirect.com/science/article/pii/S2666166721007243

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