Development and optimization of an in-house heterologous ELISA for detection of prednisolone drug in enzyme conjugates using spacers

Development and optimization of an in-house heterologous ELISA for detection of prednisolone drug in enzyme conjugates using spacers

The introduction of spacers in coating steroid protein complexes and/or enzyme conjugates or immunogens is known to exert an influence on the sensitivity of steroid enzyme immunoassays. We investigated the impact of different homobifunctional spacers, ranging in atomic length from 3 to 10, on the se...

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Bibliographic Details
Main Authors: Dinesh Kumar, Harinder Singh Oberoi, Harpal Singh, Tulsidas G. Shrivastav, Prudhvi Lal Bhukya, Mansi Kumari, Bidhan Chandra Koner, Subash Chandra Sonkar
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-08-01
Series:Frontiers in Immunology
Subjects:
heterologous ELISA
bridge/spacers
prednisolone
antibody
horseradish peroxidase
enzyme conjugates
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2023.1200328/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2023.1200328/full

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