Partial gene suppression improves identification of cancer vulnerabilities when CRISPR-Cas9 knockout is pan-lethal

Partial gene suppression improves identification of cancer vulnerabilities when CRISPR-Cas9 knockout is pan-lethal

Abstract Background Hundreds of functional genomic screens have been performed across a diverse set of cancer contexts, as part of efforts such as the Cancer Dependency Map, to identify gene dependencies—genes whose loss of function reduces cell viability or fitness. Recently, large-scale screening...

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Bibliographic Details
Main Authors: J. Michael Krill-Burger, Joshua M. Dempster, Ashir A. Borah, Brenton R. Paolella, David E. Root, Todd R. Golub, Jesse S. Boehm, William C. Hahn, James M. McFarland, Francisca Vazquez, Aviad Tsherniak
Format: Article
Language:English
Published: BMC 2023-08-01
Series:Genome Biology
Subjects:
Functional genomics
Cancer genomics
Target identification
RNAi
CRISPR-Cas systems
CRISPR-Cas9 genome editing
Online Access:https://doi.org/10.1186/s13059-023-03020-w

Internet

https://doi.org/10.1186/s13059-023-03020-w

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