Re-Use of Caco-2 Monolayers in Permeability Assays—Validation Regarding Cell Monolayer Integrity

Re-Use of Caco-2 Monolayers in Permeability Assays—Validation Regarding Cell Monolayer Integrity

Caco-2 monolayers are a common in vitro model used to evaluate human intestinal absorption. The reference protocol requires 21 days post-seeding to establish a stable and confluent cell monolayer, which is used in a single permeability assay during the period of monolayer stability (up to day 30). I...

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Bibliographic Details
Main Authors: Cristiana L. Pires, Catarina Praça, Patrícia A. T. Martins, Ana L. M. Batista de Carvalho, Lino Ferreira, Maria Paula M. Marques, Maria João Moreno
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Pharmaceutics
Subjects:
Caco-2 cells
intestinal permeability
tight junctions
lucifer yellow
high-throughput
Online Access:https://www.mdpi.com/1999-4923/13/10/1563

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https://www.mdpi.com/1999-4923/13/10/1563

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