Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum
This study was aimed at investigating the antifungal potential of basil (Ocimmum basilicum L.) extract against toxin-producing Penicillium spp. (P. aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum) isolated from food. The basil extract composition was determined by the GC-MS met...
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| Format: | Article |
| Language: | English |
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Faculty of Technology, Novi Sad
2012-01-01
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| Series: | Acta Periodica Technologica |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2012/1450-71881243247K.pdf |
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| author | Kocić-Tanackov Sunčica D. Dimić Gordana R. Pejin Dušanka J. Mojović Ljiljana V. Pejin Jelena D. Tanackov Ilija J. |
| author_facet | Kocić-Tanackov Sunčica D. Dimić Gordana R. Pejin Dušanka J. Mojović Ljiljana V. Pejin Jelena D. Tanackov Ilija J. |
| author_sort | Kocić-Tanackov Sunčica D. |
| collection | DOAJ |
| description | This study was aimed at investigating the antifungal potential of basil (Ocimmum basilicum L.) extract against toxin-producing Penicillium spp. (P. aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum) isolated from food. The basil extract composition was determined by the GC-MS method. The major component identified in the extract was estragole (86.72%). The determination of the antifungal activity of basil extract on Penicillium spp. was performed using the agar plate method. Basil extract reduced the growth of Penicillium spp. at all applied concentration levels (0.16, 0.35, 0.70, and 1.50 mL/100mL) with the colony growth inhibition from 3.6 (for P. glabrum) to 100% (for P. chrysogenum). The highest sensitivity showed P. chrysogenum, where the growth was completely inhibited at the basil extract concentration of 1.50 mL/100mL. The growth of other Penicillium spp. was partially inhibited with the colony growth inhibition of 63.4 % (P. brevicompactum), 67.5% (P. aurantiogriseum), and 71.7% (P. glabrum). Higher concentrations (0.70 and 1.50 mL/100mL) reduced the growth of the aerial mycelium of all tested Penicillium species. In addition, at the same extract concentrations, the examination of microscopic preparation showed the deformation of hyphae with the frequent occurrence of fragmentations and thickenings, occurrence of irregular vesicle, frequently without metulae and phialides, enlarged metulae. The results obtained in this investigation point to the possibility of using basil extract for the antifungal food protection. [Projekat Ministarstva nauke Republike Srbije, br. TR-31017] |
| first_indexed | 2024-12-12T03:42:35Z |
| format | Article |
| id | doaj.art-1c9b78987d454eb4b72d506f6831c60f |
| institution | Directory Open Access Journal |
| issn | 1450-7188 |
| language | English |
| last_indexed | 2024-12-12T03:42:35Z |
| publishDate | 2012-01-01 |
| publisher | Faculty of Technology, Novi Sad |
| record_format | Article |
| series | Acta Periodica Technologica |
| spelling | doaj.art-1c9b78987d454eb4b72d506f6831c60f2022-12-22T00:39:39ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882012-01-0120124324725610.2298/APT1243247KAntifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactumKocić-Tanackov Sunčica D.Dimić Gordana R.Pejin Dušanka J.Mojović Ljiljana V.Pejin Jelena D.Tanackov Ilija J.This study was aimed at investigating the antifungal potential of basil (Ocimmum basilicum L.) extract against toxin-producing Penicillium spp. (P. aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum) isolated from food. The basil extract composition was determined by the GC-MS method. The major component identified in the extract was estragole (86.72%). The determination of the antifungal activity of basil extract on Penicillium spp. was performed using the agar plate method. Basil extract reduced the growth of Penicillium spp. at all applied concentration levels (0.16, 0.35, 0.70, and 1.50 mL/100mL) with the colony growth inhibition from 3.6 (for P. glabrum) to 100% (for P. chrysogenum). The highest sensitivity showed P. chrysogenum, where the growth was completely inhibited at the basil extract concentration of 1.50 mL/100mL. The growth of other Penicillium spp. was partially inhibited with the colony growth inhibition of 63.4 % (P. brevicompactum), 67.5% (P. aurantiogriseum), and 71.7% (P. glabrum). Higher concentrations (0.70 and 1.50 mL/100mL) reduced the growth of the aerial mycelium of all tested Penicillium species. In addition, at the same extract concentrations, the examination of microscopic preparation showed the deformation of hyphae with the frequent occurrence of fragmentations and thickenings, occurrence of irregular vesicle, frequently without metulae and phialides, enlarged metulae. The results obtained in this investigation point to the possibility of using basil extract for the antifungal food protection. [Projekat Ministarstva nauke Republike Srbije, br. TR-31017]http://www.doiserbia.nb.rs/img/doi/1450-7188/2012/1450-71881243247K.pdfBasil extractantifungal activityPenicillium spp.food |
| spellingShingle | Kocić-Tanackov Sunčica D. Dimić Gordana R. Pejin Dušanka J. Mojović Ljiljana V. Pejin Jelena D. Tanackov Ilija J. Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum Acta Periodica Technologica Basil extract antifungal activity Penicillium spp. food |
| title | Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum |
| title_full | Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum |
| title_fullStr | Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum |
| title_full_unstemmed | Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum |
| title_short | Antifungal activity of the basil (Ocimmum basilicum L.) extract on Penicillium aurantiogriseum, P. glabrum, P. chrysogenum, and P. brevicompactum |
| title_sort | antifungal activity of the basil ocimmum basilicum l extract on penicillium aurantiogriseum p glabrum p chrysogenum and p brevicompactum |
| topic | Basil extract antifungal activity Penicillium spp. food |
| url | http://www.doiserbia.nb.rs/img/doi/1450-7188/2012/1450-71881243247K.pdf |
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