Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite

Salinity is among the most limiting factors of crop production worldwide. This study aims to investigate the influence of the exogenous application of zeolite, yeast, and salicylic acid in alleviating the negative effect of salt stress under field conditions. Lettuce plants (<i>Lactuca sativa&...

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Main Authors: Mahfoud Babaousmail, Mohammed S. Nili, Rania Brik, Mohammed Saadouni, Sawsan K. M. Yousif, Rihab M. Omer, Nahid A. Osman, Abdulaziz A. Alsahli, Hatem Ashour, Ahmed M. El-Taher
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Life
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Online Access:https://www.mdpi.com/2075-1729/12/10/1538
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author Mahfoud Babaousmail
Mohammed S. Nili
Rania Brik
Mohammed Saadouni
Sawsan K. M. Yousif
Rihab M. Omer
Nahid A. Osman
Abdulaziz A. Alsahli
Hatem Ashour
Ahmed M. El-Taher
author_facet Mahfoud Babaousmail
Mohammed S. Nili
Rania Brik
Mohammed Saadouni
Sawsan K. M. Yousif
Rihab M. Omer
Nahid A. Osman
Abdulaziz A. Alsahli
Hatem Ashour
Ahmed M. El-Taher
author_sort Mahfoud Babaousmail
collection DOAJ
description Salinity is among the most limiting factors of crop production worldwide. This study aims to investigate the influence of the exogenous application of zeolite, yeast, and salicylic acid in alleviating the negative effect of salt stress under field conditions. Lettuce plants (<i>Lactuca sativa</i> L. cv. Batavia) were tested in a split-plot arrangement replicated three times. The salt stress was applied as a whole-plot factor in the concentrations (0 mM, 50 mM, 100 mM, and 150 mM NaCl). After 28 days of sowing, the plants were sprayed twice during the foliage growth with (control, salicylic acid 0.02%, yeast extract 3%, and zeolite 0.5%) as a split-plot factor. The length of roots and shoots, the number and area of leaves, and the biomass accumulation (dry and fresh weights) were measured 50 days after sowing. The concentrations of total soluble sugars, proline, Chlorophylls a and b in leaves have also been quantified. Salt stress significantly reduced the growth and the total chlorophyll of the lettuce plants (<i>p</i> < 0.05) and increased their proline and sugar contents’. Zeolite application improved the growth of lettuce at 0 and 50 mM NaCl, but at the highest salinity level only the number of leaves was improved by 15%. At a mild salinity stress, the application of salicylic acid has significantly (<i>p</i> < 0.05) increased the root length, height of plant, chlorophyll, and proline contents. Regarding the high stress levels (100 and 150 mM NaCl), yeast application showed the best tolerance to salinity stress by improving significantly most of the growth parameters (<i>p</i> < 0.05) but with lower proline, sugar, and chlorophyll contents. In general, foliar spray of yeast extract may offer a good alternative source of nutrients through leaves, leading to a better tolerance of the high salt stress exerted on roots.
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spelling doaj.art-1caa7da23b484dfcaf7030ae590307a02023-11-30T22:50:21ZengMDPI AGLife2075-17292022-10-011210153810.3390/life12101538Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and ZeoliteMahfoud Babaousmail0Mohammed S. Nili1Rania Brik2Mohammed Saadouni3Sawsan K. M. Yousif4Rihab M. Omer5Nahid A. Osman6Abdulaziz A. Alsahli7Hatem Ashour8Ahmed M. El-Taher9Laboratory of Biodiversity and Biotechnology Applications in Agriculture, University of El Oued, El Oued 39000, AlgeriaLaboratory of Biodiversity and Biotechnology Applications in Agriculture, University of El Oued, El Oued 39000, AlgeriaDepartment of Agricultural Sciences, University of El Oued, El Oued 39000, AlgeriaDepartment of Agricultural Sciences, University of El Oued, El Oued 39000, AlgeriaDepartment of Chemistry, College of Arts and Science in Baljurashi, Al-Baha University, Al Bahah 65528, Saudi ArabiaDepartment of Chemistry, College of Arts and Science in Baljurashi, Al-Baha University, Al Bahah 65528, Saudi ArabiaDepartment of Science and Technology, Ranya Collage, Taif University, Taif 21944, Saudi ArabiaDepartment of Botany & Microbiology, Science College, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Agricultural Botany, Faculty of Agriculture, Ain Shams University, Cairo 11566, EgyptDepartment of Agricultural Botany, Faculty of Agriculture, Al-Azhar University, Cairo 71524, EgyptSalinity is among the most limiting factors of crop production worldwide. This study aims to investigate the influence of the exogenous application of zeolite, yeast, and salicylic acid in alleviating the negative effect of salt stress under field conditions. Lettuce plants (<i>Lactuca sativa</i> L. cv. Batavia) were tested in a split-plot arrangement replicated three times. The salt stress was applied as a whole-plot factor in the concentrations (0 mM, 50 mM, 100 mM, and 150 mM NaCl). After 28 days of sowing, the plants were sprayed twice during the foliage growth with (control, salicylic acid 0.02%, yeast extract 3%, and zeolite 0.5%) as a split-plot factor. The length of roots and shoots, the number and area of leaves, and the biomass accumulation (dry and fresh weights) were measured 50 days after sowing. The concentrations of total soluble sugars, proline, Chlorophylls a and b in leaves have also been quantified. Salt stress significantly reduced the growth and the total chlorophyll of the lettuce plants (<i>p</i> < 0.05) and increased their proline and sugar contents’. Zeolite application improved the growth of lettuce at 0 and 50 mM NaCl, but at the highest salinity level only the number of leaves was improved by 15%. At a mild salinity stress, the application of salicylic acid has significantly (<i>p</i> < 0.05) increased the root length, height of plant, chlorophyll, and proline contents. Regarding the high stress levels (100 and 150 mM NaCl), yeast application showed the best tolerance to salinity stress by improving significantly most of the growth parameters (<i>p</i> < 0.05) but with lower proline, sugar, and chlorophyll contents. In general, foliar spray of yeast extract may offer a good alternative source of nutrients through leaves, leading to a better tolerance of the high salt stress exerted on roots.https://www.mdpi.com/2075-1729/12/10/1538abiotic stressaluminosilicatebiostimilantsosmoprotectantsplant resistance<i>Saccharomyces cerevisiae</i>
spellingShingle Mahfoud Babaousmail
Mohammed S. Nili
Rania Brik
Mohammed Saadouni
Sawsan K. M. Yousif
Rihab M. Omer
Nahid A. Osman
Abdulaziz A. Alsahli
Hatem Ashour
Ahmed M. El-Taher
Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite
Life
abiotic stress
aluminosilicate
biostimilants
osmoprotectants
plant resistance
<i>Saccharomyces cerevisiae</i>
title Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite
title_full Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite
title_fullStr Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite
title_full_unstemmed Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite
title_short Improving the Tolerance to Salinity Stress in Lettuce Plants (<i>Lactuca sativa</i> L.) Using Exogenous Application of Salicylic Acid, Yeast, and Zeolite
title_sort improving the tolerance to salinity stress in lettuce plants i lactuca sativa i l using exogenous application of salicylic acid yeast and zeolite
topic abiotic stress
aluminosilicate
biostimilants
osmoprotectants
plant resistance
<i>Saccharomyces cerevisiae</i>
url https://www.mdpi.com/2075-1729/12/10/1538
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