In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control

Bovine mastitis is a complex and multifactorial disease that affects dairy herds, leading to severe economic losses to producers. Given this scenario, there is a need to find alternative methods that are effective and safe for animal and human health. The use of natural compounds derived from plant...

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Main Authors: Ida Rúbia Machado Moulin, Isabella da Costa Teixeira, Ueldiane Quintiliano Lins, Aparecida de Fátima Madella-Oliveira, Tercio da Silva de Souza
Format: Article
Language:English
Published: Instituto de Zootecnia 2022-12-01
Series:Boletim de Indústria Animal
Subjects:
Online Access:http://ojs.iz.sp.gov.br/index.php/bia/article/view/1887
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author Ida Rúbia Machado Moulin
Isabella da Costa Teixeira
Ueldiane Quintiliano Lins
Aparecida de Fátima Madella-Oliveira
Tercio da Silva de Souza
author_facet Ida Rúbia Machado Moulin
Isabella da Costa Teixeira
Ueldiane Quintiliano Lins
Aparecida de Fátima Madella-Oliveira
Tercio da Silva de Souza
author_sort Ida Rúbia Machado Moulin
collection DOAJ
description Bovine mastitis is a complex and multifactorial disease that affects dairy herds, leading to severe economic losses to producers. Given this scenario, there is a need to find alternative methods that are effective and safe for animal and human health. The use of natural compounds derived from plants is an alternative for the treatment of mastitis in dairy cows. We evaluated the in vitro antimicrobial activity of the essential oils (EO) of cinnamon (Cinnamomum sp.), melaleuca (Melaleuca alternifolia) and oregano (Origanum vulgare) against Staphylococcus aureus (ATCC 25923), aiming at future application for the control of bovine mastitis. The tests were performed in triplicate for the fixed concentration of 5 mg (50000µg disc-1) of EO. The evaluation was done by the disk diffusion method, in which trypticase soy broth (TSB) medium was inoculated with the microorganism S. aureus at 108 UFC mL-1 by pouring the medium into sterile Petri dishes with 140 mm diameter. After inoculation, sterile filter paper discs with 6 mm diameter impregnated with 5 mg of each EO were placed on the medium. After 24 h incubation at 37±1 ºC, the inhibition halos around the discs were measured. In the statistical analysis, a completely randomized design was used, in a 2 x 3, factorial scheme consisting of tea tree and eucalyptus essential oils on S. aureus strains, with 3 repetitions. The final results, determined by the arithmetic mean of the inhibition halos (mm), were submitted to analysis of variance (p<0.01) and the SNK test (p<0.05), using PROC GLM. The effect of the 5 mg concentration of oregano EO showed higher sensitivity against melaleuca EO (10.54 mm) and cinnamon EO (14.01 mm), presenting a halo of 19.09 mm. The results demonstrated that oregano essential oil has potential antibacterial activities and showed satisfactory results at a small concentration. Thus, oregano OE showed excellent action against bacterial growth. However, there is a need for further studies to evaluate its potential against standard strains such as ATCC 25923.
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spelling doaj.art-1cb43e0fa0454972a959ae3069ff09462023-08-08T23:31:24ZengInstituto de ZootecniaBoletim de Indústria Animal1981-41002022-12-0179In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis ControlIda Rúbia Machado MoulinIsabella da Costa TeixeiraUeldiane Quintiliano LinsAparecida de Fátima Madella-OliveiraTercio da Silva de Souza Bovine mastitis is a complex and multifactorial disease that affects dairy herds, leading to severe economic losses to producers. Given this scenario, there is a need to find alternative methods that are effective and safe for animal and human health. The use of natural compounds derived from plants is an alternative for the treatment of mastitis in dairy cows. We evaluated the in vitro antimicrobial activity of the essential oils (EO) of cinnamon (Cinnamomum sp.), melaleuca (Melaleuca alternifolia) and oregano (Origanum vulgare) against Staphylococcus aureus (ATCC 25923), aiming at future application for the control of bovine mastitis. The tests were performed in triplicate for the fixed concentration of 5 mg (50000µg disc-1) of EO. The evaluation was done by the disk diffusion method, in which trypticase soy broth (TSB) medium was inoculated with the microorganism S. aureus at 108 UFC mL-1 by pouring the medium into sterile Petri dishes with 140 mm diameter. After inoculation, sterile filter paper discs with 6 mm diameter impregnated with 5 mg of each EO were placed on the medium. After 24 h incubation at 37±1 ºC, the inhibition halos around the discs were measured. In the statistical analysis, a completely randomized design was used, in a 2 x 3, factorial scheme consisting of tea tree and eucalyptus essential oils on S. aureus strains, with 3 repetitions. The final results, determined by the arithmetic mean of the inhibition halos (mm), were submitted to analysis of variance (p<0.01) and the SNK test (p<0.05), using PROC GLM. The effect of the 5 mg concentration of oregano EO showed higher sensitivity against melaleuca EO (10.54 mm) and cinnamon EO (14.01 mm), presenting a halo of 19.09 mm. The results demonstrated that oregano essential oil has potential antibacterial activities and showed satisfactory results at a small concentration. Thus, oregano OE showed excellent action against bacterial growth. However, there is a need for further studies to evaluate its potential against standard strains such as ATCC 25923. http://ojs.iz.sp.gov.br/index.php/bia/article/view/1887Alternative controldairy herdsphytotherapy
spellingShingle Ida Rúbia Machado Moulin
Isabella da Costa Teixeira
Ueldiane Quintiliano Lins
Aparecida de Fátima Madella-Oliveira
Tercio da Silva de Souza
In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control
Boletim de Indústria Animal
Alternative control
dairy herds
phytotherapy
title In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control
title_full In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control
title_fullStr In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control
title_full_unstemmed In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control
title_short In Vitro Antimicrobial Activity of Essential Oils for Application in Bovine Mastitis Control
title_sort in vitro antimicrobial activity of essential oils for application in bovine mastitis control
topic Alternative control
dairy herds
phytotherapy
url http://ojs.iz.sp.gov.br/index.php/bia/article/view/1887
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