A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva

A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva

Abstract In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription droplet digital PCR (RT-ddPCR) targeting the SARS-...

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Bibliographic Details
Main Authors: Kaitlyn Wagner, Phil Fox, Elizabeth Gordon, Westen Hahn, Kenzie Olsen, Alex Markham, Dylan Buglewicz, Platon Selemenakis, Avery Lessard, Daniella Goldstein, Alissa Threatt, Luke Davis, Jake Miller-Dawson, Halie Stockett, Hailey Sanders, Kristin Rugh, Houston Turner, Michelle Remias, Maggie Williams, Jorge Chavez, Gabriel Galindo, Charlotte Cialek, Amanda Koch, Alex Fout, Bailey Fosdick, Bettina Broeckling, Mark D. Zabel
Format: Article
Language:English
Published: Nature Portfolio 2023-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-29858-5

Internet

https://doi.org/10.1038/s41598-023-29858-5

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