Effect of Single Mismatches at 3′–end of Primers on Polymerase Chain Reaction
Objective and Method – To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3•– end of a primer to amplify a 268 bp (base pair) region of the human •–globin gene using different annealing temperatures (45 to 65•C). Results – The primer with the G/T mismatch was as efficie...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Sultan Qaboos University
2000-01-01
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| Series: | Sultan Qaboos University Medical Journal |
| Subjects: | |
| Online Access: | https://journals.squ.edu.om/index.php/squmj/article/view/1188 |