Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal
In this study, chopping board meat samples collected from meat shops of Bharatpur Metropolitan City, Nepal, were screened for the presence of <i>Escherichia coli</i> (<i>E. coli</i>), with a special emphasis on the identification of extended-spectrum β-lactamase (ESBL)-produc...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Microbiology Research |
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| Online Access: | https://www.mdpi.com/2036-7481/13/4/61 |
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| author | Sagar Regmi Prem Lal Mahato Sachin Upadhayaya Hari Marasini Raju Prasad Neupane Janashrit Shrestha Rebanta Kumar Bhattarai Santosh Dhakal |
| author_facet | Sagar Regmi Prem Lal Mahato Sachin Upadhayaya Hari Marasini Raju Prasad Neupane Janashrit Shrestha Rebanta Kumar Bhattarai Santosh Dhakal |
| author_sort | Sagar Regmi |
| collection | DOAJ |
| description | In this study, chopping board meat samples collected from meat shops of Bharatpur Metropolitan City, Nepal, were screened for the presence of <i>Escherichia coli</i> (<i>E. coli</i>), with a special emphasis on the identification of extended-spectrum β-lactamase (ESBL)-producing strains. Representatives from the meat shops were also interviewed to understand the sanitary status and hygienic practices. <i>E. coli</i> bacteria were detected in one third (33/99) of the meat samples, while none of the samples had ESBL-producing strains. While 60.6% (60/99) of the meat shop personnel wore protective clothing, 15.15% (15/99) used gloves, and only 5.05% (5/99) had separate equipment for cleaning the viscera of animals. This study highlights the need for the regular screening of meat samples to identify pathogenic bacteria such as <i>E. coli</i> and for improvements in the sanitary status and hygienic practices of retail meat shops in Bharatpur Metropolitan City, Nepal. |
| first_indexed | 2024-03-09T16:04:52Z |
| format | Article |
| id | doaj.art-1cf956e25b5c469c8e10b5fe7dac15fa |
| institution | Directory Open Access Journal |
| issn | 2036-7481 |
| language | English |
| last_indexed | 2024-03-09T16:04:52Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj.art-1cf956e25b5c469c8e10b5fe7dac15fa2023-11-24T16:42:47ZengMDPI AGMicrobiology Research2036-74812022-10-0113487288110.3390/microbiolres13040061Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, NepalSagar Regmi0Prem Lal Mahato1Sachin Upadhayaya2Hari Marasini3Raju Prasad Neupane4Janashrit Shrestha5Rebanta Kumar Bhattarai6Santosh Dhakal7Faculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan 44200, NepalCollege of Tropical Agriculture and Human Resources, University of Hawai’i at Manoa, Honolulu, HI 96848, USAFaculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan 44200, NepalFaculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan 44200, NepalFaculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan 44200, NepalFaculty of Animal Science, Veterinary Science and Fisheries, Agriculture and Forestry University, Rampur, Chitwan 44200, NepalDepartment of Microbiology and Parasitology, Agriculture and Forestry University, Rampur, Chitwan 44200, NepalW. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USAIn this study, chopping board meat samples collected from meat shops of Bharatpur Metropolitan City, Nepal, were screened for the presence of <i>Escherichia coli</i> (<i>E. coli</i>), with a special emphasis on the identification of extended-spectrum β-lactamase (ESBL)-producing strains. Representatives from the meat shops were also interviewed to understand the sanitary status and hygienic practices. <i>E. coli</i> bacteria were detected in one third (33/99) of the meat samples, while none of the samples had ESBL-producing strains. While 60.6% (60/99) of the meat shop personnel wore protective clothing, 15.15% (15/99) used gloves, and only 5.05% (5/99) had separate equipment for cleaning the viscera of animals. This study highlights the need for the regular screening of meat samples to identify pathogenic bacteria such as <i>E. coli</i> and for improvements in the sanitary status and hygienic practices of retail meat shops in Bharatpur Metropolitan City, Nepal.https://www.mdpi.com/2036-7481/13/4/61antimicrobial resistance<i>Escherichia coli</i>extended-spectrum β-lactamaseslaughterhouse hygiene |
| spellingShingle | Sagar Regmi Prem Lal Mahato Sachin Upadhayaya Hari Marasini Raju Prasad Neupane Janashrit Shrestha Rebanta Kumar Bhattarai Santosh Dhakal Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal Microbiology Research antimicrobial resistance <i>Escherichia coli</i> extended-spectrum β-lactamase slaughterhouse hygiene |
| title | Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal |
| title_full | Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal |
| title_fullStr | Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal |
| title_full_unstemmed | Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal |
| title_short | Screening for <i>Escherichia coli</i> in Chopping Board Meat Samples and Survey for Sanitary and Hygienic Practices in Retail Meat Shops of Bharatpur Metropolitan City, Nepal |
| title_sort | screening for i escherichia coli i in chopping board meat samples and survey for sanitary and hygienic practices in retail meat shops of bharatpur metropolitan city nepal |
| topic | antimicrobial resistance <i>Escherichia coli</i> extended-spectrum β-lactamase slaughterhouse hygiene |
| url | https://www.mdpi.com/2036-7481/13/4/61 |
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