An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments

An in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments

Summary: Large biosynthetic gene cluster (BGC) cloning is important for discovering natural product-based drugs and remains challenging in high GC content microorganisms (e.g., Actinobacteria). Here, we present an in vitro CRISPR-Cas12a-mediated protocol for direct cloning of large DNA fragments. We...

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Bibliographic Details
Main Authors: Xiaoqian Zeng, Shuliu Wang, Mindong Liang, Weishan Wang, Yue Jiang, Fei Xu, Leshi Liu, Hao Yan, Yaojun Tong, Lixin Zhang, Gao-Yi Tan
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
CRISPR
Biotechnology and bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004021

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http://www.sciencedirect.com/science/article/pii/S2666166723004021

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