Isolation and Purification of Murine Microglial Cells for Flow Cytometry
The detailed protocol is used to isolate different cell types from murine brain as glial cells, including microglia, using an enzymatic digestion that minimizes cellular mortality. A Percoll gradient (30% to 80%) separation allows a maximal recovery of isolated murine microglial cells prior to flow...
| Asıl Yazarlar: | , , |
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| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Bio-protocol LLC
2016-01-01
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| Seri Bilgileri: | Bio-Protocol |
| Online Erişim: | https://bio-protocol.org/en/bpdetail?id=1703&type=0 |