Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria

Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteria

Abstract Background Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing...

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Bibliographic Details
Main Authors: Mathieu Gand, Bram Bloemen, Kevin Vanneste, Nancy H. C. Roosens, Sigrid C. J. De Keersmaecker
Format: Article
Language:English
Published: BMC 2023-08-01
Series:BMC Genomics
Subjects:
DNA extraction
Method evaluation
HMW DNA
Nanopore
Metagenomics
Long-read
Online Access:https://doi.org/10.1186/s12864-023-09537-5

Internet

https://doi.org/10.1186/s12864-023-09537-5

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