Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis
Objective: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing. Design: Prospective double-blinded study. Setting: Private Clinic. Patients: Couples (n...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | F&S Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666334123000466 |
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| author | Alicia L. Broussard, Ph.D., H.C.L.D. Benjamin Leader, M.D. Edna Tirado, Ph.D. Helena Russell, M.S. Hind Beydoun, Ph.D. Robert Colver, M.D. Laura Reuter, M.D. Bradford Bopp, M.D. Matthew Will, M.D. Erica Anspach Will, M.D. Glen Adaniya, Ph.D., H.C.L.D. |
| author_facet | Alicia L. Broussard, Ph.D., H.C.L.D. Benjamin Leader, M.D. Edna Tirado, Ph.D. Helena Russell, M.S. Hind Beydoun, Ph.D. Robert Colver, M.D. Laura Reuter, M.D. Bradford Bopp, M.D. Matthew Will, M.D. Erica Anspach Will, M.D. Glen Adaniya, Ph.D., H.C.L.D. |
| author_sort | Alicia L. Broussard, Ph.D., H.C.L.D. |
| collection | DOAJ |
| description | Objective: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing. Design: Prospective double-blinded study. Setting: Private Clinic. Patients: Couples (n = 150). Intervention: In vitro fertilization with preimplantation genetic testing for aneuploidy and sperm DNA fragmentation assay, as in sperm chromatin structure assay the day of retrieval. Main Outcome Measures: Laboratory outcomes are listed in the results section. Statistical analysis was performed using JMP, XYLSTAT, and STATA version 15. Results: The sperm DNA fragmentation index (DFI) in the neat ejaculate did not predict fertilization rate, quality, blastulation, or genetic diagnosis. No statistically significant results were obtained comparing <15% with >15%, <20% with >20%, <30% with >30% except for DFI. No statistically significant differences in oocyte source age or male age were observed. No statistically significant differences comparing <15% with >15%, <20% with >20%, <30% with >30% DFI at the time of standard IVF or intracytoplasmic sperm injection (ICSI) were observed for % euploid, aneuploid, mosaic, blastulation, biopsied, or D5/total biopsied. The DFI of >15% had more good quality D3 embryos than the <15% group, as did the >20% group compared with the <20% group. The ICSI fertilization was significantly higher in all 3 lower percentage groups compared with the higher counterpart. Standard IVF had significantly more blastocysts/fertilized suitable for biopsy and more D5/total number biopsied than ICSI embryos despite no difference in DFI. Conclusions: The DFI at fertilization is correlated with decreased fertilization for ICSI and IVF. |
| first_indexed | 2024-03-13T05:09:38Z |
| format | Article |
| id | doaj.art-1d4125555317487095496c85971e9c87 |
| institution | Directory Open Access Journal |
| issn | 2666-3341 |
| language | English |
| last_indexed | 2024-03-13T05:09:38Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
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| series | F&S Reports |
| spelling | doaj.art-1d4125555317487095496c85971e9c872023-06-16T05:11:50ZengElsevierF&S Reports2666-33412023-06-0142183189Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosisAlicia L. Broussard, Ph.D., H.C.L.D.0Benjamin Leader, M.D.1Edna Tirado, Ph.D.2Helena Russell, M.S.3Hind Beydoun, Ph.D.4Robert Colver, M.D.5Laura Reuter, M.D.6Bradford Bopp, M.D.7Matthew Will, M.D.8Erica Anspach Will, M.D.9Glen Adaniya, Ph.D., H.C.L.D.10Midwest Fertility Specialists, Carmel, Indiana; ReproSource, Marlborough, Massachusetts; Eastern Virginia Medical School, Norfolk, Virginia; Correspondence: Alicia L. Broussard Ph.D., H.C.L.D., Generation Next Fertility, 115 E 57th St, 11th Floor, New York, New York 10022.ReproSource, Marlborough, MassachusettsReproSource, Marlborough, MassachusettsEastern Virginia Medical School, Norfolk, VirginiaFort Belvoir Community Hospital, Fort Belvoir, VirginiaMidwest Fertility Specialists, Carmel, IndianaMidwest Fertility Specialists, Carmel, IndianaMidwest Fertility Specialists, Carmel, IndianaMidwest Fertility Specialists, Carmel, IndianaMidwest Fertility Specialists, Carmel, IndianaOvation Indianapolis, Indianapolis, IndianaObjective: To determine the effects of sperm deoxyribonucleic acid (DNA) fragmentation at the time of fertilization on in vitro fertilization (IVF) outcomes and genetic diagnosis using next generation sequencing. Design: Prospective double-blinded study. Setting: Private Clinic. Patients: Couples (n = 150). Intervention: In vitro fertilization with preimplantation genetic testing for aneuploidy and sperm DNA fragmentation assay, as in sperm chromatin structure assay the day of retrieval. Main Outcome Measures: Laboratory outcomes are listed in the results section. Statistical analysis was performed using JMP, XYLSTAT, and STATA version 15. Results: The sperm DNA fragmentation index (DFI) in the neat ejaculate did not predict fertilization rate, quality, blastulation, or genetic diagnosis. No statistically significant results were obtained comparing <15% with >15%, <20% with >20%, <30% with >30% except for DFI. No statistically significant differences in oocyte source age or male age were observed. No statistically significant differences comparing <15% with >15%, <20% with >20%, <30% with >30% DFI at the time of standard IVF or intracytoplasmic sperm injection (ICSI) were observed for % euploid, aneuploid, mosaic, blastulation, biopsied, or D5/total biopsied. The DFI of >15% had more good quality D3 embryos than the <15% group, as did the >20% group compared with the <20% group. The ICSI fertilization was significantly higher in all 3 lower percentage groups compared with the higher counterpart. Standard IVF had significantly more blastocysts/fertilized suitable for biopsy and more D5/total number biopsied than ICSI embryos despite no difference in DFI. Conclusions: The DFI at fertilization is correlated with decreased fertilization for ICSI and IVF.http://www.sciencedirect.com/science/article/pii/S2666334123000466Sperm DNA fragmentationPreimplantation genetic testingfertilizationIVF outcomesDFI |
| spellingShingle | Alicia L. Broussard, Ph.D., H.C.L.D. Benjamin Leader, M.D. Edna Tirado, Ph.D. Helena Russell, M.S. Hind Beydoun, Ph.D. Robert Colver, M.D. Laura Reuter, M.D. Bradford Bopp, M.D. Matthew Will, M.D. Erica Anspach Will, M.D. Glen Adaniya, Ph.D., H.C.L.D. Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis F&S Reports Sperm DNA fragmentation Preimplantation genetic testing fertilization IVF outcomes DFI |
| title | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
| title_full | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
| title_fullStr | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
| title_full_unstemmed | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
| title_short | Sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
| title_sort | sperm deoxyribonucleic acid fragmentation index at the time of intracytoplasmic sperm injection and standard in vitro fertilization is correlated with lower fertilization but not with blastocyst genetic diagnosis |
| topic | Sperm DNA fragmentation Preimplantation genetic testing fertilization IVF outcomes DFI |
| url | http://www.sciencedirect.com/science/article/pii/S2666334123000466 |
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