Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement
Abstract In vitro reconstruction of highly mature engineered heart tissues (EHTs) is attempted for the selection of cardiotoxic drugs suitable for individual patients before administration. Mechanical contractile force generated in the EHTs is known to be a critical indicator for evaluating the EHT...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202301831 |
| _version_ | 1797389523048464384 |
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| author | Yuta Kurashina Keisuke Fukada Shun Itai Shuichi Akizuki Ryo Sato Akari Masuda Hidenori Tani Jun Fujita Keiichi Fukuda Shugo Tohyama Hiroaki Onoe |
| author_facet | Yuta Kurashina Keisuke Fukada Shun Itai Shuichi Akizuki Ryo Sato Akari Masuda Hidenori Tani Jun Fujita Keiichi Fukuda Shugo Tohyama Hiroaki Onoe |
| author_sort | Yuta Kurashina |
| collection | DOAJ |
| description | Abstract In vitro reconstruction of highly mature engineered heart tissues (EHTs) is attempted for the selection of cardiotoxic drugs suitable for individual patients before administration. Mechanical contractile force generated in the EHTs is known to be a critical indicator for evaluating the EHT response. However, measuring contractile force requires anchoring the EHT in a tailored force‐sensing cell culture chamber, causing technical difficulties in the stable evaluation of contractile force in long‐term culture. This paper proposes a hydrogel‐sheathed human induced pluripotent stem cell (hiPSC)‐derived heart microtissue (H3M) that can provide an anchor‐free contractile force measurement platform in commonly used multi‐well plates. The contractile force associated with tissue formation and drug response is calculated by motion tracking and finite element analysis on the bending angle of the hydrogel sheath. From the experiment of the drug response, H3M is an excellent drug screening platform with high sensitivity and early testing capability compared to conventionally anchored EHT. This unique platform would be useful and versatile for regenerative therapy and drug discovery research in EHT. |
| first_indexed | 2024-03-08T22:58:12Z |
| format | Article |
| id | doaj.art-1d8a0aff19dc4f75a8977fef85d86848 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-08T22:58:12Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-1d8a0aff19dc4f75a8977fef85d868482023-12-16T04:16:13ZengWileyAdvanced Science2198-38442023-12-011035n/an/a10.1002/advs.202301831Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force MeasurementYuta Kurashina0Keisuke Fukada1Shun Itai2Shuichi Akizuki3Ryo Sato4Akari Masuda5Hidenori Tani6Jun Fujita7Keiichi Fukuda8Shugo Tohyama9Hiroaki Onoe10Department of Mechanical Engineering Faculty of Science and Technology Keio University 3‐14‐1 Hiyoshi, Kohoku‐ku Yokohama 223–8522 JapanSchool of Integrated Design Engineering Graduate School of Science and Technology Keio University 3‐14‐1 Hiyoshi, Kohoku‐ku Yokohama 223–8522 JapanDepartment of Mechanical Engineering Faculty of Science and Technology Keio University 3‐14‐1 Hiyoshi, Kohoku‐ku Yokohama 223–8522 JapanDepartment of Mechanical and Systems Engineering, School of Engineering Chukyo University 101–2 Yagoto Honmachi, Showa‐ku Nagoya Aichi 466–8666 JapanSchool of Integrated Design Engineering Graduate School of Science and Technology Keio University 3‐14‐1 Hiyoshi, Kohoku‐ku Yokohama 223–8522 JapanSchool of Integrated Design Engineering Graduate School of Science and Technology Keio University 3‐14‐1 Hiyoshi, Kohoku‐ku Yokohama 223–8522 JapanDepartment of Cardiology Keio University School of Medicine 35 Shinanomachi Shinjuku‐ku Tokyo 160–8582 JapanDepartment of Cardiology Keio University School of Medicine 35 Shinanomachi Shinjuku‐ku Tokyo 160–8582 JapanDepartment of Cardiology Keio University School of Medicine 35 Shinanomachi Shinjuku‐ku Tokyo 160–8582 JapanDepartment of Cardiology Keio University School of Medicine 35 Shinanomachi Shinjuku‐ku Tokyo 160–8582 JapanDepartment of Mechanical Engineering Faculty of Science and Technology Keio University 3‐14‐1 Hiyoshi, Kohoku‐ku Yokohama 223–8522 JapanAbstract In vitro reconstruction of highly mature engineered heart tissues (EHTs) is attempted for the selection of cardiotoxic drugs suitable for individual patients before administration. Mechanical contractile force generated in the EHTs is known to be a critical indicator for evaluating the EHT response. However, measuring contractile force requires anchoring the EHT in a tailored force‐sensing cell culture chamber, causing technical difficulties in the stable evaluation of contractile force in long‐term culture. This paper proposes a hydrogel‐sheathed human induced pluripotent stem cell (hiPSC)‐derived heart microtissue (H3M) that can provide an anchor‐free contractile force measurement platform in commonly used multi‐well plates. The contractile force associated with tissue formation and drug response is calculated by motion tracking and finite element analysis on the bending angle of the hydrogel sheath. From the experiment of the drug response, H3M is an excellent drug screening platform with high sensitivity and early testing capability compared to conventionally anchored EHT. This unique platform would be useful and versatile for regenerative therapy and drug discovery research in EHT.https://doi.org/10.1002/advs.202301831anchor‐freecontractile forceengineered heart microtissuehiPSC‐derived cardiomyocyteshydrogel |
| spellingShingle | Yuta Kurashina Keisuke Fukada Shun Itai Shuichi Akizuki Ryo Sato Akari Masuda Hidenori Tani Jun Fujita Keiichi Fukuda Shugo Tohyama Hiroaki Onoe Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement Advanced Science anchor‐free contractile force engineered heart microtissue hiPSC‐derived cardiomyocytes hydrogel |
| title | Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement |
| title_full | Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement |
| title_fullStr | Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement |
| title_full_unstemmed | Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement |
| title_short | Hydrogel‐Sheathed hiPSC‐Derived Heart Microtissue Enables Anchor‐Free Contractile Force Measurement |
| title_sort | hydrogel sheathed hipsc derived heart microtissue enables anchor free contractile force measurement |
| topic | anchor‐free contractile force engineered heart microtissue hiPSC‐derived cardiomyocytes hydrogel |
| url | https://doi.org/10.1002/advs.202301831 |
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