A cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI

The amphiphilic low-molecular-weight protein hydrophobin (HFBI) can self-assemble into a crystalline monolayer film. Here, the authors present a cryo-EM support film using such 2D crystals of hydrophobin HFBI and show that it protects specimen from the air-water interface and can help to overcome th...

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Bibliographic Details
Main Authors: Hongcheng Fan, Bo Wang, Yan Zhang, Yun Zhu, Bo Song, Haijin Xu, Yujia Zhai, Mingqiang Qiao, Fei Sun
Format: Article
Language:English
Published: Nature Portfolio 2021-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-27596-8