Direct digital sensing of protein biomarkers in solution
There are limitations with current protein sensing methods. Here the authors report DigitISA, a digital immunosensor assay based on microchip electrophoretic separation and single-molecule detection that enables quantitation of protein biomarkers in a single, solution-phase step.
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-35792-x |
| _version_ | 1811165819517796352 |
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| author | Georg Krainer Kadi L. Saar William E. Arter Timothy J. Welsh Magdalena A. Czekalska Raphaël P. B. Jacquat Quentin Peter Walther C. Traberg Arvind Pujari Akhila K. Jayaram Pavankumar Challa Christopher G. Taylor Lize-Mari van der Linden Titus Franzmann Roisin M. Owens Simon Alberti David Klenerman Tuomas P. J. Knowles |
| author_facet | Georg Krainer Kadi L. Saar William E. Arter Timothy J. Welsh Magdalena A. Czekalska Raphaël P. B. Jacquat Quentin Peter Walther C. Traberg Arvind Pujari Akhila K. Jayaram Pavankumar Challa Christopher G. Taylor Lize-Mari van der Linden Titus Franzmann Roisin M. Owens Simon Alberti David Klenerman Tuomas P. J. Knowles |
| author_sort | Georg Krainer |
| collection | DOAJ |
| description | There are limitations with current protein sensing methods. Here the authors report DigitISA, a digital immunosensor assay based on microchip electrophoretic separation and single-molecule detection that enables quantitation of protein biomarkers in a single, solution-phase step. |
| first_indexed | 2024-04-10T15:43:00Z |
| format | Article |
| id | doaj.art-1dd14b1c42c546e0b4a6295b18856433 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-10T15:43:00Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1dd14b1c42c546e0b4a6295b188564332023-02-12T12:16:39ZengNature PortfolioNature Communications2041-17232023-02-0114112110.1038/s41467-023-35792-xDirect digital sensing of protein biomarkers in solutionGeorg Krainer0Kadi L. Saar1William E. Arter2Timothy J. Welsh3Magdalena A. Czekalska4Raphaël P. B. Jacquat5Quentin Peter6Walther C. Traberg7Arvind Pujari8Akhila K. Jayaram9Pavankumar Challa10Christopher G. Taylor11Lize-Mari van der Linden12Titus Franzmann13Roisin M. Owens14Simon Alberti15David Klenerman16Tuomas P. J. Knowles17Yusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeDepartment of Chemical Engineering and Biotechnology, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeBiotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität DresdenBiotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität DresdenDepartment of Chemical Engineering and Biotechnology, University of CambridgeBiotechnology Center (BIOTEC), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universität DresdenYusuf Hamied Department of Chemistry, University of CambridgeYusuf Hamied Department of Chemistry, University of CambridgeThere are limitations with current protein sensing methods. Here the authors report DigitISA, a digital immunosensor assay based on microchip electrophoretic separation and single-molecule detection that enables quantitation of protein biomarkers in a single, solution-phase step.https://doi.org/10.1038/s41467-023-35792-x |
| spellingShingle | Georg Krainer Kadi L. Saar William E. Arter Timothy J. Welsh Magdalena A. Czekalska Raphaël P. B. Jacquat Quentin Peter Walther C. Traberg Arvind Pujari Akhila K. Jayaram Pavankumar Challa Christopher G. Taylor Lize-Mari van der Linden Titus Franzmann Roisin M. Owens Simon Alberti David Klenerman Tuomas P. J. Knowles Direct digital sensing of protein biomarkers in solution Nature Communications |
| title | Direct digital sensing of protein biomarkers in solution |
| title_full | Direct digital sensing of protein biomarkers in solution |
| title_fullStr | Direct digital sensing of protein biomarkers in solution |
| title_full_unstemmed | Direct digital sensing of protein biomarkers in solution |
| title_short | Direct digital sensing of protein biomarkers in solution |
| title_sort | direct digital sensing of protein biomarkers in solution |
| url | https://doi.org/10.1038/s41467-023-35792-x |
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