Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors
<p>Abstract</p> <p>Hoxa9 is a homeodomain transcription factor important for the generation of Flt3+hiIL-7R- lymphoid biased-multipotential progenitors, Flt3+IL-7R+ common lymphoid progenitors (CLPs), and B cell precursors (BCP) in bone marrow (BM). In addition to B-cell, Flt3+IL-7...
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| Format: | Article |
| Language: | English |
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BMC
2013-01-01
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| Series: | BMC Immunology |
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| Online Access: | http://www.biomedcentral.com/1471-2172/14/5 |
| _version_ | 1818580157645979648 |
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| author | Gwin Kimberly Dolence Joseph J Shapiro Mariya B Medina Kay L |
| author_facet | Gwin Kimberly Dolence Joseph J Shapiro Mariya B Medina Kay L |
| author_sort | Gwin Kimberly |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Hoxa9 is a homeodomain transcription factor important for the generation of Flt3+hiIL-7R- lymphoid biased-multipotential progenitors, Flt3+IL-7R+ common lymphoid progenitors (CLPs), and B cell precursors (BCP) in bone marrow (BM). In addition to B-cell, Flt3+IL-7R+ CLPs possess NK and DC developmental potentials, although DCs arise from Flt3+IL-7R- myeloid progenitors as well. In this study, we investigated the requirement for Hoxa9, from Flt3+ or Flt3- progenitor subsets, in the development of NK and DC lineage cells in BM. Flt3+IL-7R+Ly6D- CLPs and their Flt3+IL-7R+Ly6D+ B lineage-restricted progeny (BLP) were significantly reduced in <it>hoxa9−/−</it> mice. Interestingly, the reduction in Flt3+IL-7R+ CLPs in <it>hoxa9−/−</it> mice had no impact on the generation of NK precursor (NKP) subsets, the differentiation of NKP into mature NK cells, or NK homeostasis. Similarly, percentages and numbers of common dendritic progenitors (CDP), as well as their plasmacytoid or conventional dendritic cell progeny in <it>hoxa9−/−</it> mice were comparable to wildtype. These findings reveal distinct requirements for Hoxa9 or Hoxa9/Flt3 molecular circuits in regulation of B versus NK and DC development in BM.</p> |
| first_indexed | 2024-12-16T07:13:08Z |
| format | Article |
| id | doaj.art-1dd8b66020514436b53b8657e1fc2d1a |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-16T07:13:08Z |
| publishDate | 2013-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-1dd8b66020514436b53b8657e1fc2d1a2022-12-21T22:39:51ZengBMCBMC Immunology1471-21722013-01-01141510.1186/1471-2172-14-5Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitorsGwin KimberlyDolence Joseph JShapiro Mariya BMedina Kay L<p>Abstract</p> <p>Hoxa9 is a homeodomain transcription factor important for the generation of Flt3+hiIL-7R- lymphoid biased-multipotential progenitors, Flt3+IL-7R+ common lymphoid progenitors (CLPs), and B cell precursors (BCP) in bone marrow (BM). In addition to B-cell, Flt3+IL-7R+ CLPs possess NK and DC developmental potentials, although DCs arise from Flt3+IL-7R- myeloid progenitors as well. In this study, we investigated the requirement for Hoxa9, from Flt3+ or Flt3- progenitor subsets, in the development of NK and DC lineage cells in BM. Flt3+IL-7R+Ly6D- CLPs and their Flt3+IL-7R+Ly6D+ B lineage-restricted progeny (BLP) were significantly reduced in <it>hoxa9−/−</it> mice. Interestingly, the reduction in Flt3+IL-7R+ CLPs in <it>hoxa9−/−</it> mice had no impact on the generation of NK precursor (NKP) subsets, the differentiation of NKP into mature NK cells, or NK homeostasis. Similarly, percentages and numbers of common dendritic progenitors (CDP), as well as their plasmacytoid or conventional dendritic cell progeny in <it>hoxa9−/−</it> mice were comparable to wildtype. These findings reveal distinct requirements for Hoxa9 or Hoxa9/Flt3 molecular circuits in regulation of B versus NK and DC development in BM.</p>http://www.biomedcentral.com/1471-2172/14/5Hoxa9Flt3IL-7RB-cellNK-cellDCDevelopment |
| spellingShingle | Gwin Kimberly Dolence Joseph J Shapiro Mariya B Medina Kay L Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors BMC Immunology Hoxa9 Flt3 IL-7R B-cell NK-cell DC Development |
| title | Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors |
| title_full | Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors |
| title_fullStr | Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors |
| title_full_unstemmed | Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors |
| title_short | Differential requirement for Hoxa9 in the development and differentiation of B, NK, and DC-lineage cells from Flt3+ multipotential progenitors |
| title_sort | differential requirement for hoxa9 in the development and differentiation of b nk and dc lineage cells from flt3 multipotential progenitors |
| topic | Hoxa9 Flt3 IL-7R B-cell NK-cell DC Development |
| url | http://www.biomedcentral.com/1471-2172/14/5 |
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