Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics

Many software suites and spectral libraries have been developed for DIA proteomics data analysis. Here, the authors create benchmark data sets to evaluate four commonly used software tools combined with seven spectral libraries in both global proteomics and phosphoproteomics analysis.

Bibliographic Details
Main Authors: Ronghui Lou, Ye Cao, Shanshan Li, Xiaoyu Lang, Yunxia Li, Yaoyang Zhang, Wenqing Shui
Format: Article
Language:English
Published: Nature Portfolio 2023-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-35740-1
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author Ronghui Lou
Ye Cao
Shanshan Li
Xiaoyu Lang
Yunxia Li
Yaoyang Zhang
Wenqing Shui
author_facet Ronghui Lou
Ye Cao
Shanshan Li
Xiaoyu Lang
Yunxia Li
Yaoyang Zhang
Wenqing Shui
author_sort Ronghui Lou
collection DOAJ
description Many software suites and spectral libraries have been developed for DIA proteomics data analysis. Here, the authors create benchmark data sets to evaluate four commonly used software tools combined with seven spectral libraries in both global proteomics and phosphoproteomics analysis.
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spelling doaj.art-1ddc8e335f7b40868b2f2f3f64f66a602023-01-08T12:16:18ZengNature PortfolioNature Communications2041-17232023-01-0114111710.1038/s41467-022-35740-1Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomicsRonghui Lou0Ye Cao1Shanshan Li2Xiaoyu Lang3Yunxia Li4Yaoyang Zhang5Wenqing Shui6iHuman Institute, ShanghaiTech UniversityUniversity of Chinese Academy of SciencesiHuman Institute, ShanghaiTech UniversityiHuman Institute, ShanghaiTech UniversityInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesiHuman Institute, ShanghaiTech UniversityMany software suites and spectral libraries have been developed for DIA proteomics data analysis. Here, the authors create benchmark data sets to evaluate four commonly used software tools combined with seven spectral libraries in both global proteomics and phosphoproteomics analysis.https://doi.org/10.1038/s41467-022-35740-1
spellingShingle Ronghui Lou
Ye Cao
Shanshan Li
Xiaoyu Lang
Yunxia Li
Yaoyang Zhang
Wenqing Shui
Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics
Nature Communications
title Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics
title_full Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics
title_fullStr Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics
title_full_unstemmed Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics
title_short Benchmarking commonly used software suites and analysis workflows for DIA proteomics and phosphoproteomics
title_sort benchmarking commonly used software suites and analysis workflows for dia proteomics and phosphoproteomics
url https://doi.org/10.1038/s41467-022-35740-1
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