A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing

A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing

Abstract Background Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use...

Full description

Bibliographic Details
Main Authors: François F. Barbier, Tinashe G. Chabikwa, Muhammad U. Ahsan, Stacey E. Cook, Rosanna Powell, Milos Tanurdzic, Christine A. Beveridge
Format: Article
Language:English
Published: BMC 2019-06-01
Series:Plant Methods
Subjects:
DNA RNA extraction
CTAB
SDS
Woody species
Macadamia
Avocado
Online Access:http://link.springer.com/article/10.1186/s13007-019-0447-3

Internet

http://link.springer.com/article/10.1186/s13007-019-0447-3

Similar Items