Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats
Background: Cisplatin (CP) is a chemotherapy drug, with the major side effect of nephrotoxicity. The level of endothelin-1 (ET-1) increases during nephrotoxicity, which is accompanied with vasoconstrictive properties. Bosentan (BOS) is a nonselective ET-1 receptor antagonist, having vasodilatory and...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=83;epage=83;aulast=Jokar |
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| author | Zahra Jokar Mehdi Nematbakhsh Maryam Moeini Ardeshir Talebi |
| author_facet | Zahra Jokar Mehdi Nematbakhsh Maryam Moeini Ardeshir Talebi |
| author_sort | Zahra Jokar |
| collection | DOAJ |
| description | Background: Cisplatin (CP) is a chemotherapy drug, with the major side effect of nephrotoxicity. The level of endothelin-1 (ET-1) increases during nephrotoxicity, which is accompanied with vasoconstrictive properties. Bosentan (BOS) is a nonselective ET-1 receptor antagonist, having vasodilatory and anti-hypertension effects. The purpose of this study was to investigate the renoprotective effect of BOS against CP-induced nephrotoxicity in male and female rats.
Materials and Methods: Male and female rats were divided into six groups; groups 1-3 and 4-6 were male and female rats, respectively. Animals in groups 1 and 4 were considered as negative control and groups 2 and 5 considered as positive control groups received BOS (30 mg/kg/day) alone and CP (2.5 mg/kg/day) alone, respectively, for 1-week. The animals in groups 3 and 6 were treated with both CP and BOS. Finally, serum parameters were measured, and the kidney tissue was subjected to staining to evaluate tissue damage.
Results: The serum levels of blood urea nitrogen and creatinine, kidney tissue damage score and kidney weight elevated, and body weight significantly decreased in both CP alone and in CP plus BOS-treated groups when compared with the control groups (P < 0.05), while BOS did not ameliorate these parameters neither in males nor in females. No significant differences were observed in serum levels of nitrite and malondialdehyde between the groups, but kidney tissue level of nitrite decreased significantly in CP alone and CP plus BOS-treated groups (P < 0.05).
Conclusion: Renoprotective effect of BOS, as ET-1 blocker, was not observed against CP-induced nephrotoxicity neither in male nor in female rats. This is while BOS promoted the severity of injuries in females. |
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| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-12-24T03:47:21Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-1e24f91c370d43f2bdaf2ef0c015505b2022-12-21T17:16:41ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752015-01-0141838310.4103/2277-9175.156642Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female ratsZahra JokarMehdi NematbakhshMaryam MoeiniArdeshir TalebiBackground: Cisplatin (CP) is a chemotherapy drug, with the major side effect of nephrotoxicity. The level of endothelin-1 (ET-1) increases during nephrotoxicity, which is accompanied with vasoconstrictive properties. Bosentan (BOS) is a nonselective ET-1 receptor antagonist, having vasodilatory and anti-hypertension effects. The purpose of this study was to investigate the renoprotective effect of BOS against CP-induced nephrotoxicity in male and female rats. Materials and Methods: Male and female rats were divided into six groups; groups 1-3 and 4-6 were male and female rats, respectively. Animals in groups 1 and 4 were considered as negative control and groups 2 and 5 considered as positive control groups received BOS (30 mg/kg/day) alone and CP (2.5 mg/kg/day) alone, respectively, for 1-week. The animals in groups 3 and 6 were treated with both CP and BOS. Finally, serum parameters were measured, and the kidney tissue was subjected to staining to evaluate tissue damage. Results: The serum levels of blood urea nitrogen and creatinine, kidney tissue damage score and kidney weight elevated, and body weight significantly decreased in both CP alone and in CP plus BOS-treated groups when compared with the control groups (P < 0.05), while BOS did not ameliorate these parameters neither in males nor in females. No significant differences were observed in serum levels of nitrite and malondialdehyde between the groups, but kidney tissue level of nitrite decreased significantly in CP alone and CP plus BOS-treated groups (P < 0.05). Conclusion: Renoprotective effect of BOS, as ET-1 blocker, was not observed against CP-induced nephrotoxicity neither in male nor in female rats. This is while BOS promoted the severity of injuries in females.http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=83;epage=83;aulast=JokarBosentancisplatingendernephrotoxicityrat |
| spellingShingle | Zahra Jokar Mehdi Nematbakhsh Maryam Moeini Ardeshir Talebi Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats Advanced Biomedical Research Bosentan cisplatin gender nephrotoxicity rat |
| title | Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats |
| title_full | Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats |
| title_fullStr | Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats |
| title_full_unstemmed | Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats |
| title_short | Role of endothelin-1 antagonist; bosentan, against cisplatin-induced nephrotoxicity in male and female rats |
| title_sort | role of endothelin 1 antagonist bosentan against cisplatin induced nephrotoxicity in male and female rats |
| topic | Bosentan cisplatin gender nephrotoxicity rat |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=83;epage=83;aulast=Jokar |
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