Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos

Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos

The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present stud...

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Bibliographic Details
Main Authors: L.E. Alvares, A. Mantoani, J.E. Corrente, L.L. Coutinho
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2003-12-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Quantitative RT-PCR
Standard-curve
Myogenic regulatory factors
Gene expression
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004

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