Direct bisulphite conversion of cervical samples for DNA methylation analysis

Direct bisulphite conversion of cervical samples for DNA methylation analysis

Sodium bisulphite conversion of DNA to separate methylated from unmethylated cytosines is a standard for methylation analysis. This study evaluated a direct cell conversion protocol on cervical samples as alternative to isolated genomic DNA as input. Clinician-collected cervical samples (n = 120) we...

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Bibliographic Details
Main Authors: Lisanne Verhoef, A. N Floore, Saskia Doorn, Kate Cuschieri, Ramya Bhatia, A. T Hesselink, Chris J.L.M. Meijer, Renske D.M. Steenbergen, Daniëlle A.M. Heideman
Format: Article
Language:English
Published: Taylor & Francis Group 2022-10-01
Series:Epigenetics
Subjects:
sodium bisulphite treatment
high-throughput
host-cell dna methylation
hpv triage
cervical screening
liquid-based cytology
Online Access:http://dx.doi.org/10.1080/15592294.2021.1992911

Internet

http://dx.doi.org/10.1080/15592294.2021.1992911

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