Single base mutations in the nucleocapsid gene of SARS-CoV-2 affects amplification efficiency of sequence variants and may lead to assay failure

Single base mutations in the nucleocapsid gene of SARS-CoV-2 affects amplification efficiency of sequence variants and may lead to assay failure

Reverse transcriptase quantitative PCR (RT-qPCR) is the main diagnostic assay used to detect SARS-CoV-2 RNA in respiratory samples. RT-qPCR is performed by specifically targeting the viral genome using complementary oligonucleotides called primers and probes. This approach relies on prior knowledge...

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Bibliographic Details
Main Authors: Nathaniel Storey, Julianne R Brown, Rui P A Pereira, Denise M O'Sullivan, Jim F Huggett, Rachel Williams, Judith Breuer, Kathryn A Harris
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:Journal of Clinical Virology Plus
Subjects:
SARS-CoV-2
Nucleocapsid
Mutations
PCR
Failure
Silico
Online Access:http://www.sciencedirect.com/science/article/pii/S2667038021000296

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http://www.sciencedirect.com/science/article/pii/S2667038021000296

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