Characterization of antibody-antigen interactions using biolayer interferometry

Summary: This protocol describes the use of a biolayer interferometry platform for assessing antibody-antigen interactions. The protocol focuses on affinity determination and epitope binning, although the system can be utilized for measuring any protein-protein interaction. Readings are collected in...

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Bibliographic Details
Main Authors: Tal Noy-Porat, Ron Alcalay, Adva Mechaly, Eldar Peretz, Efi Makdasi, Ronit Rosenfeld, Ohad Mazor
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005426