Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis
Collagen is significantly upregulated in colorectal liver metastasis (CRLM) compared to liver tissue. Expression levels of specific collagen types in CRLM resemble those in colorectal cancer (CRC) and colon tissue. We investigated whether the collagen hydroxylation pattern from the primary tumor als...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.557737/full |
| _version_ | 1819206476420349952 |
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| author | Nick A. van Huizen Nick A. van Huizen Peter C. Burgers Joost van Rosmalen Michail Doukas Jan N. M. IJzermans Theo M. Luider |
| author_facet | Nick A. van Huizen Nick A. van Huizen Peter C. Burgers Joost van Rosmalen Michail Doukas Jan N. M. IJzermans Theo M. Luider |
| author_sort | Nick A. van Huizen |
| collection | DOAJ |
| description | Collagen is significantly upregulated in colorectal liver metastasis (CRLM) compared to liver tissue. Expression levels of specific collagen types in CRLM resemble those in colorectal cancer (CRC) and colon tissue. We investigated whether the collagen hydroxylation pattern from the primary tumor also migrates with the metastatic tumor. The degree of collagen alpha-1(I) hydroxylation in colon, CRC, liver, and CRLM tissue of the same individuals (n = 14) was studied with mass spectrometry. The degree of hydroxylation was investigated in 36 collagen alpha-1(I) peptides, covering 54% of the triple helical region. The degree of hydroxylation in liver tissue was similar to that in colon tissue. The overall degree of hydroxylation was significantly lower (9 ± 14%) in CRC tissue and also significantly lower (12 ± 22%) in CRLM tissue compared to colon. Furthermore, eleven peptides with a specific number of hydroxylations are significantly different between CRLM and liver tissue; these peptides could be candidates for the detection of CRLM. For one of these eleven peptides, a matching naturally occurring peptide in urine has been identified as being significantly different between patients suffering from CRLM and healthy controls. The hydroxylation pattern in CRLM resembles partly the pattern in liver, primary colorectal cancer and colon. |
| first_indexed | 2024-12-23T05:08:12Z |
| format | Article |
| id | doaj.art-1e9e20ccf378436dab2e2acb48fb98f0 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-23T05:08:12Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-1e9e20ccf378436dab2e2acb48fb98f02022-12-21T17:59:01ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-09-011010.3389/fonc.2020.557737557737Down-Regulation of Collagen Hydroxylation in Colorectal Liver MetastasisNick A. van Huizen0Nick A. van Huizen1Peter C. Burgers2Joost van Rosmalen3Michail Doukas4Jan N. M. IJzermans5Theo M. Luider6Department of Surgery, Erasmus University Medical Center, Rotterdam, NetherlandsDepartment of Neurology, Erasmus University Medical Center, Rotterdam, NetherlandsDepartment of Neurology, Erasmus University Medical Center, Rotterdam, NetherlandsDepartment of Biostatistics, Erasmus University Medical Center, Rotterdam, NetherlandsDepartment of Pathology, Erasmus University Medical Center, Rotterdam, NetherlandsDepartment of Surgery, Erasmus University Medical Center, Rotterdam, NetherlandsDepartment of Neurology, Erasmus University Medical Center, Rotterdam, NetherlandsCollagen is significantly upregulated in colorectal liver metastasis (CRLM) compared to liver tissue. Expression levels of specific collagen types in CRLM resemble those in colorectal cancer (CRC) and colon tissue. We investigated whether the collagen hydroxylation pattern from the primary tumor also migrates with the metastatic tumor. The degree of collagen alpha-1(I) hydroxylation in colon, CRC, liver, and CRLM tissue of the same individuals (n = 14) was studied with mass spectrometry. The degree of hydroxylation was investigated in 36 collagen alpha-1(I) peptides, covering 54% of the triple helical region. The degree of hydroxylation in liver tissue was similar to that in colon tissue. The overall degree of hydroxylation was significantly lower (9 ± 14%) in CRC tissue and also significantly lower (12 ± 22%) in CRLM tissue compared to colon. Furthermore, eleven peptides with a specific number of hydroxylations are significantly different between CRLM and liver tissue; these peptides could be candidates for the detection of CRLM. For one of these eleven peptides, a matching naturally occurring peptide in urine has been identified as being significantly different between patients suffering from CRLM and healthy controls. The hydroxylation pattern in CRLM resembles partly the pattern in liver, primary colorectal cancer and colon.https://www.frontiersin.org/article/10.3389/fonc.2020.557737/fullpost-translational modificationmass spectrometrycolorectal cancercolorectal liver metastasiscollagenhydroxyproline |
| spellingShingle | Nick A. van Huizen Nick A. van Huizen Peter C. Burgers Joost van Rosmalen Michail Doukas Jan N. M. IJzermans Theo M. Luider Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis Frontiers in Oncology post-translational modification mass spectrometry colorectal cancer colorectal liver metastasis collagen hydroxyproline |
| title | Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis |
| title_full | Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis |
| title_fullStr | Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis |
| title_full_unstemmed | Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis |
| title_short | Down-Regulation of Collagen Hydroxylation in Colorectal Liver Metastasis |
| title_sort | down regulation of collagen hydroxylation in colorectal liver metastasis |
| topic | post-translational modification mass spectrometry colorectal cancer colorectal liver metastasis collagen hydroxyproline |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.557737/full |
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