Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference

Interrogating the Role of the Two Distinct Fructose-Bisphosphate Aldolases of Bacillus methanolicus by Site-Directed Mutagenesis of Key Amino Acids and Gene Repression by CRISPR Interference

The Gram-positive Bacillus methanolicus shows plasmid-dependent methylotrophy. This facultative ribulose monophosphate (RuMP) cycle methylotroph possesses two fructose bisphosphate aldolases (FBA) with distinct kinetic properties. The chromosomally encoded FBAC is the major glycolytic aldolase. The...

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Bibliographic Details
Main Authors: Kerstin Schultenkämper, Desirée D. Gütle, Marina Gil López, Laura B. Keller, Lin Zhang, Oliver Einsle, Jean-Pierre Jacquot, Volker F. Wendisch
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Microbiology
Subjects:
transketolase
methylotrophy
glycolysis
gluconeogenesis
CRISPR interference
fructose-1,6-bisphosphate aldolase
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.669220/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.669220/full

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