Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a

CRISPR/Cas12a-based detection is a novel approach for the efficient, sequence-specific identification of viruses. Here we adopt the use of CRISPR/Cas12a to identify the tomato brown rugose fruit virus (ToBRFV), a new and emerging tobamovirus which is causing substantial damage to the global tomato i...

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Bibliographic Details
Main Authors: Dan Mark Alon, Hagit Hak, Menachem Bornstein, Gur Pines, Ziv Spiegelman
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Plants
Subjects:
Online Access:https://www.mdpi.com/2223-7747/10/6/1256