Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase

Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase

Until 2015, loss-of-function studies to elucidate protein function in Leishmania relied on gene disruption through homologous recombination. Then, the CRISPR/Cas9 revolution reached these protozoan parasites allowing efficient genome editing with one round of transfection. In addition, the developme...

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Bibliographic Details
Main Authors: Caroline R. Espada, José Carlos Quilles, Andreia Albuquerque-Wendt, Mario C. Cruz, Tom Beneke, Lucas B. Lorenzon, Eva Gluenz, Angela K. Cruz, Silvia R. B. Uliana
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
CRISPR/Cas9
Leishmania braziliensis
PF16
endogenous tagging
knockout
reverse genetics
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.772311/full

Internet

https://www.frontiersin.org/articles/10.3389/fcimb.2021.772311/full

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