A Synthetic Biology Workflow Reveals Variation in Processing and Solubility of Nitrogenase Proteins Targeted to Plant Mitochondria, and Differing Tolerance of Targeting Sequences in a Bacterial Nitrogenase Assay

A Synthetic Biology Workflow Reveals Variation in Processing and Solubility of Nitrogenase Proteins Targeted to Plant Mitochondria, and Differing Tolerance of Targeting Sequences in a Bacterial Nitrogenase Assay

While industrial nitrogen fertilizer is intrinsic to modern agriculture, it is expensive and environmentally harmful. One approach to reduce fertilizer usage is to engineer the bacterial nitrogenase enzyme complex within plant mitochondria, a location that may support enzyme function. Our current st...

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Bibliographic Details
Main Authors: Shoko Okada, Christina M. Gregg, Robert Silas Allen, Amratha Menon, Dawar Hussain, Vanessa Gillespie, Ema Johnston, Keren Byrne, Michelle Lisa Colgrave, Craig C. Wood
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-09-01
Series:Frontiers in Plant Science
Subjects:
nitrogenase
plant mitochondria
mitochondrial targeting
mitochondrial processing peptidase
protein solubility
Klebsiella
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2020.552160/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2020.552160/full

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