Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation
The in vitro digestion and fermentation behaviors of <i>Lactobacillus helveticus</i> LZ-R-5- and <i>L</i><i>. pentosus</i> LZ-R-17-sourced exopolysaccharides (LHEPS and LPEPS) were investigated by stimulated batch-culture fermentation system. The results illustrat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/16/2501 |
| _version_ | 1797432145132650496 |
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| author | Mengjia Xu Zhi Li Xiaogan Zhao Wei Li |
| author_facet | Mengjia Xu Zhi Li Xiaogan Zhao Wei Li |
| author_sort | Mengjia Xu |
| collection | DOAJ |
| description | The in vitro digestion and fermentation behaviors of <i>Lactobacillus helveticus</i> LZ-R-5- and <i>L</i><i>. pentosus</i> LZ-R-17-sourced exopolysaccharides (LHEPS and LPEPS) were investigated by stimulated batch-culture fermentation system. The results illustrated that LHEPS was resistant to simulated saliva and gastrointestinal (GSI) digestion, whereas LPEPS generated a few monosaccharides after digestion without significant influence on its main structure. Additionally, LHEPS and LPEPS could be consumed by the human gut microbiota and presented stronger bifidogenic effect comparing to α-glucan and β-glucan, as they promote the proliferation of <i>Lactobacillus</i> and <i>Bifidobacterium</i> in cultures and exhibited high values of selectivity index (13.88 and 11.78, respectively). Furthermore, LPEPS achieved higher contents of lactic acid and acetic acid (35.74 mM and 45.91 mM, respectively) than LHEPS (35.20 mM and 44.65 mM, respectively) during fermentation for 48 h, thus also resulting in a larger amount of total SCFAs (110.86 mM). These results have clearly indicated the potential prebiotic property of EPS fractions from <i>L</i><i>. helveticus</i> LZ-R-5 and <i>L</i><i>. pentosus</i> LZ-R-17, which could be further developed as new functional food prebiotics to beneficially improve human gut health. |
| first_indexed | 2024-03-09T09:57:01Z |
| format | Article |
| id | doaj.art-1ef5dc51f981415fb73210a4f10437cd |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T09:57:01Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-1ef5dc51f981415fb73210a4f10437cd2023-12-01T23:42:41ZengMDPI AGFoods2304-81582022-08-011116250110.3390/foods11162501Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and FermentationMengjia Xu0Zhi Li1Xiaogan Zhao2Wei Li3State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaThe in vitro digestion and fermentation behaviors of <i>Lactobacillus helveticus</i> LZ-R-5- and <i>L</i><i>. pentosus</i> LZ-R-17-sourced exopolysaccharides (LHEPS and LPEPS) were investigated by stimulated batch-culture fermentation system. The results illustrated that LHEPS was resistant to simulated saliva and gastrointestinal (GSI) digestion, whereas LPEPS generated a few monosaccharides after digestion without significant influence on its main structure. Additionally, LHEPS and LPEPS could be consumed by the human gut microbiota and presented stronger bifidogenic effect comparing to α-glucan and β-glucan, as they promote the proliferation of <i>Lactobacillus</i> and <i>Bifidobacterium</i> in cultures and exhibited high values of selectivity index (13.88 and 11.78, respectively). Furthermore, LPEPS achieved higher contents of lactic acid and acetic acid (35.74 mM and 45.91 mM, respectively) than LHEPS (35.20 mM and 44.65 mM, respectively) during fermentation for 48 h, thus also resulting in a larger amount of total SCFAs (110.86 mM). These results have clearly indicated the potential prebiotic property of EPS fractions from <i>L</i><i>. helveticus</i> LZ-R-5 and <i>L</i><i>. pentosus</i> LZ-R-17, which could be further developed as new functional food prebiotics to beneficially improve human gut health.https://www.mdpi.com/2304-8158/11/16/2501<i>Lactobacillus helveticus</i> LZ-R-5<i>L</i><i>. pentosus</i> LZ-R-17exopolysaccharidesin vitro digestion and fermentationselectivity indexprebiotic |
| spellingShingle | Mengjia Xu Zhi Li Xiaogan Zhao Wei Li Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation Foods <i>Lactobacillus helveticus</i> LZ-R-5 <i>L</i><i>. pentosus</i> LZ-R-17 exopolysaccharides in vitro digestion and fermentation selectivity index prebiotic |
| title | Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation |
| title_full | Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation |
| title_fullStr | Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation |
| title_full_unstemmed | Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation |
| title_short | Prebiotic Properties of Exopolysaccharides from <i>Lactobacillus helveticus</i> LZ-R-5 and <i>L. pentosus</i> LZ-R-17 Evaluated by In Vitro Simulated Digestion and Fermentation |
| title_sort | prebiotic properties of exopolysaccharides from i lactobacillus helveticus i lz r 5 and i l pentosus i lz r 17 evaluated by in vitro simulated digestion and fermentation |
| topic | <i>Lactobacillus helveticus</i> LZ-R-5 <i>L</i><i>. pentosus</i> LZ-R-17 exopolysaccharides in vitro digestion and fermentation selectivity index prebiotic |
| url | https://www.mdpi.com/2304-8158/11/16/2501 |
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