Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease
The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the...
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MDPI AG
2021-06-01
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| author | A-Hyeon Lee Sung-Chul Hong Inwook Park Soljee Yoon YoungSoo Kim Jinsik Kim Seung-Hoon Yang |
| author_facet | A-Hyeon Lee Sung-Chul Hong Inwook Park Soljee Yoon YoungSoo Kim Jinsik Kim Seung-Hoon Yang |
| author_sort | A-Hyeon Lee |
| collection | DOAJ |
| description | The visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the microalgae <i>Phaeodactylum tricornutum</i> has fluorescent excitation and emission wavelengths without any interference for Aβ interaction. Thus, we applied it to monitor Aβ aggregation in AD transgenic mouse models. Aβ plaques were visualized using fucoxanthin in the brain tissue of APP/PS1 and 5×FAD mice by histological staining with different staining methods. By comparing fucoxanthin-positive and thioflavin S-positive stained regions in the brains, we found that they are colocalized and that fucoxanthin can detect Aβ aggregates. Our finding suggests that fucoxanthin from <i>P. tricornutum</i> can be a new Aβ fluorescent imaging reagent in AD diagnosis. |
| first_indexed | 2024-03-09T04:55:50Z |
| format | Article |
| id | doaj.art-1f02009e7258414c993325d2ebbdd4dc |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T04:55:50Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
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| spelling | doaj.art-1f02009e7258414c993325d2ebbdd4dc2023-12-03T13:05:24ZengMDPI AGApplied Sciences2076-34172021-06-011113587810.3390/app11135878Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s DiseaseA-Hyeon Lee0Sung-Chul Hong1Inwook Park2Soljee Yoon3YoungSoo Kim4Jinsik Kim5Seung-Hoon Yang6Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, KoreaNatural Informatics Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, KoreaDepartment of Pharmacy, Department of Integrative Biotechnology & Translational Medicine, and Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 21983, KoreaDepartment of Pharmacy, Department of Integrative Biotechnology & Translational Medicine, and Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 21983, KoreaDepartment of Pharmacy, Department of Integrative Biotechnology & Translational Medicine, and Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 21983, KoreaDepartment of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, KoreaDepartment of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, KoreaThe visualization of misfolded Aβ peptides by using fluorescence chemical dyes is very important in Alzheimer’s disease (AD) diagnosis. Here, we describe the fluorescent substance, fucoxanthin, which detects Aβ aggregates in the brain of AD transgenic mouse models. We found that fucoxanthin from the microalgae <i>Phaeodactylum tricornutum</i> has fluorescent excitation and emission wavelengths without any interference for Aβ interaction. Thus, we applied it to monitor Aβ aggregation in AD transgenic mouse models. Aβ plaques were visualized using fucoxanthin in the brain tissue of APP/PS1 and 5×FAD mice by histological staining with different staining methods. By comparing fucoxanthin-positive and thioflavin S-positive stained regions in the brains, we found that they are colocalized and that fucoxanthin can detect Aβ aggregates. Our finding suggests that fucoxanthin from <i>P. tricornutum</i> can be a new Aβ fluorescent imaging reagent in AD diagnosis.https://www.mdpi.com/2076-3417/11/13/5878fucoxanthinAlzheimer’s diseaseamyloid plaquefluorescent dyeAD diagnosis |
| spellingShingle | A-Hyeon Lee Sung-Chul Hong Inwook Park Soljee Yoon YoungSoo Kim Jinsik Kim Seung-Hoon Yang Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease Applied Sciences fucoxanthin Alzheimer’s disease amyloid plaque fluorescent dye AD diagnosis |
| title | Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease |
| title_full | Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease |
| title_fullStr | Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease |
| title_full_unstemmed | Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease |
| title_short | Validation of Fucoxanthin from Microalgae <em>Phaeodactylum tricornutum</em> for the Detection of Amyloid Burden in Transgenic Mouse Models of Alzheimer’s Disease |
| title_sort | validation of fucoxanthin from microalgae em phaeodactylum tricornutum em for the detection of amyloid burden in transgenic mouse models of alzheimer s disease |
| topic | fucoxanthin Alzheimer’s disease amyloid plaque fluorescent dye AD diagnosis |
| url | https://www.mdpi.com/2076-3417/11/13/5878 |
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