The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components
Midecamycin is a 16-membered macrolide antibiotic. It can inhibit the synthesis of bacterial proteins by blocking up the activity of peptidyl transferase in the 50S ribosome. We used high-resolution mass spectrometry to analyze midecamycin, and quantitatively analyzed of each component of midecamyci...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/9/8/225 |
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| author | Mingyan Yu Weijian Wang Wenkun Liu Huiru Yang Tong Sun Haiyun Zhao Dejun Chen |
| author_facet | Mingyan Yu Weijian Wang Wenkun Liu Huiru Yang Tong Sun Haiyun Zhao Dejun Chen |
| author_sort | Mingyan Yu |
| collection | DOAJ |
| description | Midecamycin is a 16-membered macrolide antibiotic. It can inhibit the synthesis of bacterial proteins by blocking up the activity of peptidyl transferase in the 50S ribosome. We used high-resolution mass spectrometry to analyze midecamycin, and quantitatively analyzed of each component of midecamycin produced by 18 different companies. The developed methods were validated by assessing linearity, limit of quantitation (LOQ), accuracy, precision, and robustness. Good separations were achieved for all components. Ten components of midecamycin were identified, and the contents of these components were determined in midecamycin produced by different companies. The demarcation between impurities and components of midecamycin was not clear. A ligand-docking model was used for predicting the impurities and components of midecamycin. Components and impurities were docked with the target. The results reported in this article may be important for quality control and the predictive demarcation between impurities and components of midecamycin. |
| first_indexed | 2024-03-09T03:50:12Z |
| format | Article |
| id | doaj.art-1f0d52d9470c4dd5becbbe9b67bc21cd |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T03:50:12Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Separations |
| spelling | doaj.art-1f0d52d9470c4dd5becbbe9b67bc21cd2023-12-03T14:28:12ZengMDPI AGSeparations2297-87392022-08-019822510.3390/separations9080225The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and ComponentsMingyan Yu0Weijian Wang1Wenkun Liu2Huiru Yang3Tong Sun4Haiyun Zhao5Dejun Chen6Shandong Institute for Food and Drug Control, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaMidecamycin is a 16-membered macrolide antibiotic. It can inhibit the synthesis of bacterial proteins by blocking up the activity of peptidyl transferase in the 50S ribosome. We used high-resolution mass spectrometry to analyze midecamycin, and quantitatively analyzed of each component of midecamycin produced by 18 different companies. The developed methods were validated by assessing linearity, limit of quantitation (LOQ), accuracy, precision, and robustness. Good separations were achieved for all components. Ten components of midecamycin were identified, and the contents of these components were determined in midecamycin produced by different companies. The demarcation between impurities and components of midecamycin was not clear. A ligand-docking model was used for predicting the impurities and components of midecamycin. Components and impurities were docked with the target. The results reported in this article may be important for quality control and the predictive demarcation between impurities and components of midecamycin.https://www.mdpi.com/2297-8739/9/8/225macrolidemidecamycinhigh-resolution mass spectrometry (HRMS)molecular docking |
| spellingShingle | Mingyan Yu Weijian Wang Wenkun Liu Huiru Yang Tong Sun Haiyun Zhao Dejun Chen The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components Separations macrolide midecamycin high-resolution mass spectrometry (HRMS) molecular docking |
| title | The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components |
| title_full | The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components |
| title_fullStr | The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components |
| title_full_unstemmed | The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components |
| title_short | The Quality Control of Midecamycin and the Predictive Demarcation between Its Impurities and Components |
| title_sort | quality control of midecamycin and the predictive demarcation between its impurities and components |
| topic | macrolide midecamycin high-resolution mass spectrometry (HRMS) molecular docking |
| url | https://www.mdpi.com/2297-8739/9/8/225 |
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