Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
The lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells (LECs) in LNs draining naïve and inflamed skin. We found that sub...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/6/1371 |
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| author | Eliane Sibler Yuliang He Luca Ducoli Nadja Keller Noriki Fujimoto Lothar C. Dieterich Michael Detmar |
| author_facet | Eliane Sibler Yuliang He Luca Ducoli Nadja Keller Noriki Fujimoto Lothar C. Dieterich Michael Detmar |
| author_sort | Eliane Sibler |
| collection | DOAJ |
| description | The lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells (LECs) in LNs draining naïve and inflamed skin. We found that subsets of LECs lining the different LN sinuses responded individually to skin inflammation, suggesting that they exert distinct functions under pathological conditions. Among the genes dysregulated during inflammation, we confirmed an up-regulation of CD200 in the LECs lining the subcapsular sinus floor with a possible function in immune regulation. Furthermore, by in silico analysis, we predicted numerous possible interactions of LECs with diverse immune cells in the LNs and found similarities in the transcriptional changes of LN LECs in different skin inflammation settings. In summary, we provide an in-depth analysis of the transcriptional landscape of LN LECs in the naïve state and in skin inflammation. |
| first_indexed | 2024-03-10T10:46:34Z |
| format | Article |
| id | doaj.art-1f31922249cc4d20950912dccf87468d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T10:46:34Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-1f31922249cc4d20950912dccf87468d2023-11-21T22:32:37ZengMDPI AGCells2073-44092021-06-01106137110.3390/cells10061371Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph NodesEliane Sibler0Yuliang He1Luca Ducoli2Nadja Keller3Noriki Fujimoto4Lothar C. Dieterich5Michael Detmar6Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 3, 8093 Zürich, SwitzerlandInstitute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 3, 8093 Zürich, SwitzerlandInstitute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 3, 8093 Zürich, SwitzerlandInstitute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 3, 8093 Zürich, SwitzerlandDepartment of Dermatology, Shiga University of Medical Science, Otsu 520-2192, JapanInstitute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 3, 8093 Zürich, SwitzerlandInstitute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Vladimir-Prelog-Weg 3, 8093 Zürich, SwitzerlandThe lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells (LECs) in LNs draining naïve and inflamed skin. We found that subsets of LECs lining the different LN sinuses responded individually to skin inflammation, suggesting that they exert distinct functions under pathological conditions. Among the genes dysregulated during inflammation, we confirmed an up-regulation of CD200 in the LECs lining the subcapsular sinus floor with a possible function in immune regulation. Furthermore, by in silico analysis, we predicted numerous possible interactions of LECs with diverse immune cells in the LNs and found similarities in the transcriptional changes of LN LECs in different skin inflammation settings. In summary, we provide an in-depth analysis of the transcriptional landscape of LN LECs in the naïve state and in skin inflammation.https://www.mdpi.com/2073-4409/10/6/1371scRNA-seqinflammationlymph nodelymphatic endothelial cells |
| spellingShingle | Eliane Sibler Yuliang He Luca Ducoli Nadja Keller Noriki Fujimoto Lothar C. Dieterich Michael Detmar Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes Cells scRNA-seq inflammation lymph node lymphatic endothelial cells |
| title | Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes |
| title_full | Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes |
| title_fullStr | Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes |
| title_full_unstemmed | Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes |
| title_short | Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes |
| title_sort | single cell transcriptional heterogeneity of lymphatic endothelial cells in normal and inflamed murine lymph nodes |
| topic | scRNA-seq inflammation lymph node lymphatic endothelial cells |
| url | https://www.mdpi.com/2073-4409/10/6/1371 |
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