Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
Summary: Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programmi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004537 |
| _version_ | 1827890648378769408 |
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| author | Razan Sheta Maxime Teixeira Walid Idi Abid Oueslati |
| author_facet | Razan Sheta Maxime Teixeira Walid Idi Abid Oueslati |
| author_sort | Razan Sheta |
| collection | DOAJ |
| description | Summary: Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks.For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-12T21:13:36Z |
| format | Article |
| id | doaj.art-1f3285d140484f579b21e06dacfc5a3f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-12T21:13:36Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-1f3285d140484f579b21e06dacfc5a3f2023-07-30T04:22:58ZengElsevierSTAR Protocols2666-16672023-09-0143102486Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neuronsRazan Sheta0Maxime Teixeira1Walid Idi2Abid Oueslati3CHU de Québec Research Center, Axe Neurosciences, Quebec City, QC, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, QC, Canada; Corresponding authorCHU de Québec Research Center, Axe Neurosciences, Quebec City, QC, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, QC, CanadaCHU de Québec Research Center, Axe Neurosciences, Quebec City, QC, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, QC, CanadaCHU de Québec Research Center, Axe Neurosciences, Quebec City, QC, Canada; Department of Molecular Medicine, Faculty of Medicine, Université Laval, Quebec City, QC, Canada; Corresponding authorSummary: Generation of functional human dopaminergic (DA) neurons from human induced pluripotent stem cells (hiPSCs) is a crucial tool for modeling dopamine-related human diseases and cell replacement therapies. Here, we present a protocol to combine neuralizing transcription factor (NGN2) programming and DA patterning to differentiate hiPSCs into mature and functional induced DA (iDA) neurons. We describe steps from transduction of hiPSCs and neural induction through to differentiation and maturation of near-pure, fully functional iDA neurons within 3 weeks.For complete details on the use and execution of this protocol, please refer to Sheta et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723004537NeuroscienceStem CellsCell Differentiation |
| spellingShingle | Razan Sheta Maxime Teixeira Walid Idi Abid Oueslati Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons STAR Protocols Neuroscience Stem Cells Cell Differentiation |
| title | Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons |
| title_full | Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons |
| title_fullStr | Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons |
| title_full_unstemmed | Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons |
| title_short | Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons |
| title_sort | optimized protocol for the generation of functional human induced pluripotent stem cell derived dopaminergic neurons |
| topic | Neuroscience Stem Cells Cell Differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723004537 |
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