Ancestral mutations as a tool for solubilizing proteins: The case of a hydrophobic phosphate-binding protein

Ancestral mutations as a tool for solubilizing proteins: The case of a hydrophobic phosphate-binding protein

Stable and soluble proteins are ideal candidates for functional and structural studies. Unfortunately, some proteins or enzymes can be difficult to isolate, being sometimes poorly expressed in heterologous systems, insoluble and/or unstable. Numerous methods have been developed to address these issu...

Full description

Bibliographic Details
Main Authors: Daniel Gonzalez, Julien Hiblot, Nune Darbinian, Jernelle C. Miller, Guillaume Gotthard, Shohreh Amini, Eric Chabriere, Mikael Elias
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:FEBS Open Bio
Subjects:
Ancestral librairies
Protein engineering
Protein solubilization
Hydrophobic proteins
Phosphate-binding proteins
DING proteins
Online Access:http://www.sciencedirect.com/science/article/pii/S2211546313000806

Internet

http://www.sciencedirect.com/science/article/pii/S2211546313000806

Similar Items