Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR

Efficient bi-allelic tagging in human induced pluripotent stem cells using CRISPR

Summary: Allelic tagging of endogenous genes enables studying gene function and transcriptional control in the native genomic context. Here, we present an efficient protocol for bi-allelic tagging of protein-coding genes with fluorescent reporters in human iPSCs using the CRISPR-Cas9-mediated homolo...

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Bibliographic Details
Main Authors: Xingjie Ren, Maya Asami Takagi, Yin Shen
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell Culture
Genetics
Molecular Biology
CRISPR
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000424

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723000424

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