Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification
In vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37....
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Sciendo
2017-07-01
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Series: | Contemporary Agriculture |
Subjects: | |
Online Access: | https://doi.org/10.1515/contagri-2017-0008 |