Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification
In vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2017-07-01
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| Series: | Contemporary Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/contagri-2017-0008 |
| _version_ | 1818390436972068864 |
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| author | Vujović Tatjana Ružić Đurđina Cerović Radosav |
| author_facet | Vujović Tatjana Ružić Đurđina Cerović Radosav |
| author_sort | Vujović Tatjana |
| collection | DOAJ |
| description | In vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40 min at room temperature with the PVS3 solution (glycerol 50% and sucrose 50%). They were subsequently frozen in individual microdroplets of vitrification solution, by direct immersion in liquid nitrogen (LN), and kept therein for 2, 4, 8 and 24 h. The explant rewarming was performed in an unloading solution (0.8 M sucrose) for 30 min at room temperature. The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions. The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively. However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants. Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls, which fully confirms the purpose of cryopreservation in the long-term preservation of plant material. |
| first_indexed | 2024-12-14T04:57:36Z |
| format | Article |
| id | doaj.art-1f9fadc337284bf4adf6c2ee54f66715 |
| institution | Directory Open Access Journal |
| issn | 2466-4774 |
| language | English |
| last_indexed | 2024-12-14T04:57:36Z |
| publishDate | 2017-07-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Contemporary Agriculture |
| spelling | doaj.art-1f9fadc337284bf4adf6c2ee54f667152022-12-21T23:16:18ZengSciendoContemporary Agriculture2466-47742017-07-01661-2445010.1515/contagri-2017-0008contagri-2017-0008Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet VitrificationVujović Tatjana0Ružić Đurđina1Cerović Radosav2Fruit Research Institute, Kralja Petra I no. 9, 32000Čačak, Republic of SerbiaFruit Research Institute, Kralja Petra I no. 9, 32000Čačak, Republic of SerbiaUniversity of Belgrade, Innovation Center, Faculty of Technology and Metallurgy, Karnegijeva 4, 11120Belgrade, Republic of SerbiaIn vitro shoot tips of the blackberry cultivar ‘Čačanska Bestrna’ were cryopreserved using the droplet vitrification technique. Upon loading (30 min) in a solution of 1.9 M glycerol and 0.5 M sucrose, the explants were dehydrated for 40 min on ice with the PVS A3 vitrification solution (glycerol 37.5%, dimethyl sulfoxide 15%, ethylene glycol 15% and sucrose 22.5%) and for 40 min at room temperature with the PVS3 solution (glycerol 50% and sucrose 50%). They were subsequently frozen in individual microdroplets of vitrification solution, by direct immersion in liquid nitrogen (LN), and kept therein for 2, 4, 8 and 24 h. The explant rewarming was performed in an unloading solution (0.8 M sucrose) for 30 min at room temperature. The duration of LN exposure did not exert significant effects on the survival and regrowth of explants in both types of vitrification solutions. The survival and regrowth of cryopreserved shoot tips dehydrated with PVS3 solution ranged between 90–95% and 80–90%, respectively. However, dehydration with PVS A3 resulted in a lower survival rate (80–90%) and a considerably lower regrowth rate (55–65%) of explants. Monitoring the shoots regenerated in the in vitro culture revealed their normal capacity for multiplication and rooting in comparison with the controls, which fully confirms the purpose of cryopreservation in the long-term preservation of plant material.https://doi.org/10.1515/contagri-2017-0008blackberryin vitro cryopreservationvitrification solutionsliquid nitrogen |
| spellingShingle | Vujović Tatjana Ružić Đurđina Cerović Radosav Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification Contemporary Agriculture blackberry in vitro cryopreservation vitrification solutions liquid nitrogen |
| title | Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification |
| title_full | Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification |
| title_fullStr | Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification |
| title_full_unstemmed | Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification |
| title_short | Effect of the Duration of Liquid Nitrogen Storage on the Regrowth of Blackberry Cryopreserved by Droplet Vitrification |
| title_sort | effect of the duration of liquid nitrogen storage on the regrowth of blackberry cryopreserved by droplet vitrification |
| topic | blackberry in vitro cryopreservation vitrification solutions liquid nitrogen |
| url | https://doi.org/10.1515/contagri-2017-0008 |
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