Engineering the conserved and noncatalytic residues of a thermostable β-1,4-endoglucanase to improve specific activity and thermostability
Abstract Endoglucanases are increasingly applied in agricultural and industrial applications as a key biocatalyst for cellulose biodegradation. However, the low performance in extreme conditions seriously challenges the enzyme’s commercial utilization. To obtain endoglucanases with substantially imp...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2018-02-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-018-21246-8 |