Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion
Several studies indicate that cholesterol esterification is deregulated in cancers. The present study aimed to characterize the role of cholesterol esterification in proliferation and invasion of two tumor cells expressing an activated cholecystokinin 2 receptor (CCK2R). A significant increase in ch...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2009-11-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520306866 |
| _version_ | 1818899318963175424 |
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| author | Michael R. Paillasse Philippe de Medina Guillaume Amouroux Loubna Mhamdi Marc Poirot Sandrine Silvente-Poirot |
| author_facet | Michael R. Paillasse Philippe de Medina Guillaume Amouroux Loubna Mhamdi Marc Poirot Sandrine Silvente-Poirot |
| author_sort | Michael R. Paillasse |
| collection | DOAJ |
| description | Several studies indicate that cholesterol esterification is deregulated in cancers. The present study aimed to characterize the role of cholesterol esterification in proliferation and invasion of two tumor cells expressing an activated cholecystokinin 2 receptor (CCK2R). A significant increase in cholesterol esterification and activity of Acyl-CoA:cholesterol acyltransferase (ACAT) was measured in tumor cells expressing a constitutively activated oncogenic mutant of the CCK2R (CCK2R-E151A cells) compared with nontumor cells expressing the wild-type CCK2R (CCK2R-WT cells). Inhibition of cholesteryl ester formation and ACAT activity by Sah58-035, an inhibitor of ACAT, decreased by 34% and 73% CCK2R-E151A cell growth and invasion. Sustained activation of CCK2R-WT cells by gastrin increased cholesteryl ester production while addition of cholesteryl oleate to the culture medium of CCK2R-WT cells increased cell proliferation and invasion to a level close to that of CCK2R-E151A cells. In U87 glioma cells, a model of autocrine growth stimulation of the CCK2R, inhibition of cholesterol esterification and ACAT activity by Sah58-035 and two selective antagonists of the CCK2R significantly reduced cell proliferation and invasion. In both models, cholesteryl ester formation was found dependent on protein kinase zeta/ extracellular signal-related kinase 1/2 (PKCζ/ERK1/2) activation. These results show that signaling through ACAT/cholesterol esterification is a novel pathway for the CCK2R that contributes to tumor cell proliferation and invasion. |
| first_indexed | 2024-12-19T19:46:04Z |
| format | Article |
| id | doaj.art-201f055a2fd3429d8913d0b637d58c79 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T19:46:04Z |
| publishDate | 2009-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-201f055a2fd3429d8913d0b637d58c792022-12-21T20:08:07ZengElsevierJournal of Lipid Research0022-22752009-11-01501122032211Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasionMichael R. Paillasse0Philippe de Medina1Guillaume Amouroux2Loubna Mhamdi3Marc Poirot4Sandrine Silvente-Poirot5INSERM 563, Equipe Métabolisme, Oncogenèse et Différenciation cellulaire, Centre de Physiopathologie de Toulouse Purpan, Institut Claudius Regaud, Toulouse France; Affichem, Toulouse, FranceINSERM 563, Equipe Métabolisme, Oncogenèse et Différenciation cellulaire, Centre de Physiopathologie de Toulouse Purpan, Institut Claudius Regaud, Toulouse France; Affichem, Toulouse, FranceINSERM 563, Equipe Métabolisme, Oncogenèse et Différenciation cellulaire, Centre de Physiopathologie de Toulouse Purpan, Institut Claudius Regaud, Toulouse FranceINSERM 563, Equipe Métabolisme, Oncogenèse et Différenciation cellulaire, Centre de Physiopathologie de Toulouse Purpan, Institut Claudius Regaud, Toulouse France; Affichem, Toulouse, FranceINSERM 563, Equipe Métabolisme, Oncogenèse et Différenciation cellulaire, Centre de Physiopathologie de Toulouse Purpan, Institut Claudius Regaud, Toulouse France; Université Toulouse III Paul Sabatier, Toulouse, FranceTo whom correspondence should be addressed; INSERM 563, Equipe Métabolisme, Oncogenèse et Différenciation cellulaire, Centre de Physiopathologie de Toulouse Purpan, Institut Claudius Regaud, Toulouse France; Université Toulouse III Paul Sabatier, Toulouse, FranceSeveral studies indicate that cholesterol esterification is deregulated in cancers. The present study aimed to characterize the role of cholesterol esterification in proliferation and invasion of two tumor cells expressing an activated cholecystokinin 2 receptor (CCK2R). A significant increase in cholesterol esterification and activity of Acyl-CoA:cholesterol acyltransferase (ACAT) was measured in tumor cells expressing a constitutively activated oncogenic mutant of the CCK2R (CCK2R-E151A cells) compared with nontumor cells expressing the wild-type CCK2R (CCK2R-WT cells). Inhibition of cholesteryl ester formation and ACAT activity by Sah58-035, an inhibitor of ACAT, decreased by 34% and 73% CCK2R-E151A cell growth and invasion. Sustained activation of CCK2R-WT cells by gastrin increased cholesteryl ester production while addition of cholesteryl oleate to the culture medium of CCK2R-WT cells increased cell proliferation and invasion to a level close to that of CCK2R-E151A cells. In U87 glioma cells, a model of autocrine growth stimulation of the CCK2R, inhibition of cholesterol esterification and ACAT activity by Sah58-035 and two selective antagonists of the CCK2R significantly reduced cell proliferation and invasion. In both models, cholesteryl ester formation was found dependent on protein kinase zeta/ extracellular signal-related kinase 1/2 (PKCζ/ERK1/2) activation. These results show that signaling through ACAT/cholesterol esterification is a novel pathway for the CCK2R that contributes to tumor cell proliferation and invasion.http://www.sciencedirect.com/science/article/pii/S0022227520306866cholesteryl esteracyl-CoA:cholesterol acyltransferaseconstitutively active mutantautocrine stimulationG protein coupled receptorgastrin receptor |
| spellingShingle | Michael R. Paillasse Philippe de Medina Guillaume Amouroux Loubna Mhamdi Marc Poirot Sandrine Silvente-Poirot Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion Journal of Lipid Research cholesteryl ester acyl-CoA:cholesterol acyltransferase constitutively active mutant autocrine stimulation G protein coupled receptor gastrin receptor |
| title | Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion |
| title_full | Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion |
| title_fullStr | Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion |
| title_full_unstemmed | Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion |
| title_short | Signaling through cholesterol esterification: a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion |
| title_sort | signaling through cholesterol esterification a new pathway for the cholecystokinin 2 receptor involved in cell growth and invasion |
| topic | cholesteryl ester acyl-CoA:cholesterol acyltransferase constitutively active mutant autocrine stimulation G protein coupled receptor gastrin receptor |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520306866 |
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