Specific N-terminal amino acids potentiate the periplasmic expression of single-chain variable fragments in Escherichia coli
Single-chain variable fragments (ScFvs) are important in therapy, diagnosis and research because of their elevated antigen affinity and low immunogenicity. At present, high-yield scFv expression in Escherichia coli is limited by insoluble aggregation in the reducing environment of the cytoplasm or l...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-02-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0107 |